| 不同产地夏枯草中抗非小细胞肺癌成分科罗索酸的差异比较 |
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| 引用本文： | 欧阳慧,封亮,徐友华,王珊珊,王汝上,朱荃,项平.不同产地夏枯草中抗非小细胞肺癌成分科罗索酸的差异比较[J].中华中医药杂志,2012(6):1571-1574. |
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| 作者姓名： | 欧阳慧 封亮 徐友华 王珊珊 王汝上 朱荃 项平 |
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| 作者单位： | 澳门科技大学国家中药质量控制重点实验室中医药生物技术实验室;广州康臣药业有限公司肾病药物研究中心 |
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| 基金项目： | 澳门科技发展基金会项目(No.050)~~ |
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| 摘 要： | 目的:比较不同产地夏枯草中抗非小细胞肺癌活性成分科罗索酸的含量差异。方法:通过四甲基偶氮唑蓝(MTT)和吖啶橙(AO)/溴化乙啶(EB),评价科罗索酸对非小细胞肺癌细胞的增殖抑制以及凋亡作用;建立高效液相色谱法,比较14个产地夏枯草中科罗索酸的含量差异。结果:科罗索酸抑制SPC-A-1人非小细胞肺癌的生长增殖(IC50=1.05×10-6mol/L),并且诱导SPC-A-1细胞凋亡。14个产地夏枯草中科罗索酸的含量差异较大,山西产地的含量最高,河南-2产地的含量最低。结论:科罗索酸为夏枯草中的抗非小细胞肺癌的活性成分,不同产地夏枯草的科罗索酸含量差异较大。
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| 关 键 词： | 夏枯草 科罗索酸 四甲基偶氮唑蓝 凋亡 高效液相色谱法 产地 质量 |
Comparision of anti-non small cell lung cancer compound corosolic acid in Prunella vulgaris L.from different regions |
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| OUYANG Hui,FENG Liang,XU You-hua,WANG Shan-shan,WANG Ru-shang,ZHU Quan,XIANG Ping.Comparision of anti-non small cell lung cancer compound corosolic acid in Prunella vulgaris L.from different regions[J].China Journal of Traditional Chinese Medicine and Pharmacy,2012(6):1571-1574. |
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| Authors: | OUYANG Hui FENG Liang XU You-hua WANG Shan-shan WANG Ru-shang ZHU Quan XIANG Ping |
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| Institution: | 1 (1State Key Laboratory of Quality Research in Chinese Medicine,Biotechnology Labortory of Chinese Medicine,Macau University of Science and Technology,Macau 999078,China;2Institute of Conson Co.for Chinese Medicine in Kidiney Diseases,Consun Pharmaceutical Group,Guangzhou 510530,China) |
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| Abstract: | Objective: To compare the content difference of anti-non small cell lung cancer active ingredient corosolic acid in Prunella vulgaris L.from different regions.Methods:MTT and acridine orange(AO)-ethidium bromide(EB) methods were established to evaluate anti-proliferation and apoptosis activity.A high performance liquid chromatography method was established for this comparision.Results: Corosolic acid inhibited SPC-A-1 cell proliferation and IC50 was 1.05×10-6mol/L.Corosolic acid also induced SPC-A-1 cell apoptosis.There was obvious differences on the content of corosolic acid in Prunella vulgaris L.from 14 different regions.Shanxi had the highest content of corosolic acid while Henan-2 had the lowest.Conclusion: Corosolic acid was a anti-non small cell lung cancer active ingredient in Prunella vulgaris L.The content of corosolic acid in Prunella vulgaris L.from different regions has a significant difference on the market. |
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| Keywords: | Prunella vulgaris L Corosolic acid MTT Apoptosis High performance liquid chromatography Region Quality |
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