| 高效液相色谱法测定肾石通颗粒中槲皮素与山柰素含量 |
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| 引用本文： | 王义恩.高效液相色谱法测定肾石通颗粒中槲皮素与山柰素含量[J].中国药业,2010,19(7):27-28. |
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| 作者姓名： | 王义恩 |
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| 作者单位： | 浙江省舟山市嵊泗县中医院,浙江,舟山,202450 |
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| 摘 要： | 目的建立肾石通颗粒中槲皮素与山柰素的含量测定方法。方法采用AgilentExtend—C18柱（250mm×4．6mm，5μm），以甲醇-0．4％磷酸溶液（47：53）为流动相，检测波长为360nm，流速1．0mL／min，柱温为25℃。结果槲皮素与山柰素质量浓度分别在0．90。9．04μg／mL和1．96—19．62μg／mL范围内与峰面积线性关系良好（r分别为0．9992和0．9993），平均回收率分别为97．33％和95．69％，RSD分别为1．04％和0．87％（n=9）。结论该方法专属性强、灵敏度高、重现性好、操作简便，适用于肾石通颗粒的质量控制。
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| 关 键 词： | 肾石通颗粒 槲皮素 山柰素 高效液相色谱法 |
Content Determination of Quercetin and Kaempferol in Shenshitong Granules by HPLC |
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| Wang Yien.Content Determination of Quercetin and Kaempferol in Shenshitong Granules by HPLC[J].China Pharmaceuticals,2010,19(7):27-28. |
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| Authors: | Wang Yien |
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| Institution: | Wang Yien (Shengsi County Hospital of Traditional Chinese Medicine, Zhoushan, Zhefiang, China 202450) |
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| Abstract: | Objective To establish a HPLC method for the determination of quercetin and. kaempferol in Shenshitong Granules. Methods The HPLC analysis was performed on the Agilent Extend-C18 column（250 mm×4.6 mm, 5 μm） with methanol-0.4% phosphoric acid （47：53） as the mobile phase. The detective wavelength was at 360 nm, the flow rate was 1.0 mL/min. Results Quercetin in the concentrations of 0. 90 -9. 04 μg/mL showed the good linearity（ r =0. 999 2）, the average recovery was rate was 97.33%, RSD = 1.04% （ n =9）; kaempferol in the concentrations of 1.96- 19. 62 μg/mL showed good the linearity （r =0. 999 3）, the average recovery rate was 95. 69%, RSD = 0. 87% （n =9）. Conclusion The method is simple, sensitive and reliable with good reproducibility, which can be used for the quality control of this preparation. |
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| Keywords: | Shenshitong Granules quercetin kaempferol HPLC |
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