| 某地区麻疹病毒核蛋白及血凝素基因序列分析 |
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| 引用本文： | 何梅英,陈伟红,陈传德,刘卫民,卓菲.某地区麻疹病毒核蛋白及血凝素基因序列分析[J].现代预防医学,2005,32(4):295-296. |
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| 作者姓名： | 何梅英 陈伟红 陈传德 刘卫民 卓菲 |
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| 作者单位： | 深圳市罗湖区疾病预防控制中心,深圳,518020 |
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| 基金项目： | 深圳市卫生科技立项 (2 0 0 2 0 41 6 6 ) |
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| 摘 要： | 目的：探讨深圳市麻疹病毒的基因型别和特征。方法：采用B95a细胞分离培养麻疹病毒，通过PT-PCR方法扩增麻疹病毒核蛋白N末端450bp片段和H基因合序列并测序、分析和基因库Genebank中麻疹的各代表株基因序列的同源性进行比较。结果：分离到1株麻疹病毒，N基因与H1基因型代表株相比同源性为98．4％，H基因与H1基因代表株同源性达99．5％。结论：深圳市麻疹流行株属于H1基因型。
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| 关 键 词： | 麻疹病毒 基因序列分析 血凝素 PT-PCR方法 H1基因型 白及 细胞分离培养 同源性 代表株 基因型别 深圳市 H基因 N末端 核蛋白 基因库 N基因 流行株 |
| 文章编号： | 1003-8507(2005)04-0295-02 |
| 修稿时间： | 2004年12月17 |
SEQUENCE ANALYSIS ON NUCLEOPOTEIN GENE AND HEMAGGLUTININ GENE OF MEASLES VIRUS ISOLATED IN SHENZHEN |
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| HE Mei-ying,CHEN Wei-hong,CHEN Chuan-de,et al..SEQUENCE ANALYSIS ON NUCLEOPOTEIN GENE AND HEMAGGLUTININ GENE OF MEASLES VIRUS ISOLATED IN SHENZHEN[J].Modern Preventive Medicine,2005,32(4):295-296. |
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| Authors: | HE Mei-ying CHEN Wei-hong CHEN Chuan-de |
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| Institution: | HE Mei-ying,CHEN Wei-hong,CHEN Chuan-de,et al.Center for Disease Control and Prevention of Luohu District,Shenzhen,518020. |
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| Abstract: | Objective:To study the genetic character and genotype of measles in Shenzhen district.Methods:Throat swabs were collected for virus isolation with B95a cells. A 450 bp fragment of the C-terminal of the nucleoprotein(N) gene and hemagglutinin (H) gene were amplified by RT-PCR and subjected to sequence and phylogenetic analysis using DNASTAR software.Results:Only one strain was isolated from 15 throat-swabs, N genes homogeneity was 98.4% and H genes was 99.5% compared with other wild-type viruses of H1 genotype.Conclusion:Shenzhen circulating measles viruses were belong to clade H1. |
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| Keywords: | Measles virus Nucleoprotein gene Hemagglutinin gene Genotype Sequence analysis |
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