2010年合肥儿童手足口病病例肠道病毒血清基因型分析

目的:对合肥地区2010年5~7月儿童手足口病流行期间的手足口病患儿进行病原学调查。方法:采集手足口病患儿的疱疹液、粪便、咽拭子进行病毒分离;分离的病毒接种非洲绿猴肾细胞(Vero)后待细胞病变(CPE)达到++++以上后提取RNA。分别用EV71和CA16的VP1基因的特异性引物进行RT-PCR鉴定,并根据血清型不同比较其临床特征。结果:77例患儿采集的标本中65例分离到病毒;经RT-PCR检测发现其中47例CA16阳性,18例为EV71,比例为2.6∶1。CA16感染和EV71感染在患者的年龄大小、症状轻重等方面无显著统计学差异。结论:手足口病患儿检测到的主要病原是CA16和EV71,两者感染对临床的影响无显著差异。

Detection of enterovirus from children with hand, foot and mouth disease in Hefei 2010

Objective：It was noticed that coxsackievirusA16 （CA16） and enterovirus 71 （EV71） were two major etiological agents of hand, foot and mouth disease （HFMD） in children. This study was to investigate the major causes of an HFMD epidemic in children in Hefei from April to June of 2010. Methods ： Specimens （ including vesicle flu- id, stool and throat swabs） were collected from 77 patientswith HFMD. The specimens were inoculated into Vero and /or RD cells. After CPE was over ＋＋＋＋＋,RNA was extracted and detected by RT-PCR assay with two specific primer pairs for VP1 genes of EV71 and CA16 respectively. The EV71 and CA16 were identified depending on the size of PCR products. Results： Viruses were isolated in specimens from 65 patients. RT-PCR results indicated that 47 pa- tients were identified as positive for CA16 and 18 patients were identified as positive for EVT1, the ratio between CA16 and EV71 was 2.6：1.There were no significant difference showed on patients＇s gender, age and clinical symp- toms between these two virus infection. Conclusion,＂ CA16 and EV71 were the primary causes of HFMD during the epidemic and no significant difference of clinical manifestation caused by these 2 virus.