静磁场对大鼠牙周膜组织中骨形成蛋白-2表达的影响

目的:观察静磁场对大鼠牙周膜组织中骨形成蛋白(BMP-2)的影响,探讨磁场对牙周膜组织的作用机制。方法:35只健康雌性Wistet大鼠,随机分为对照组、实验对照组和实验组。在实验对照组和实验组的大鼠双侧颊部,分别埋入未充磁的磁体和表面磁场强度为0.12 T的磁体,于术后第2、4、7天,切取双侧上颌磨牙及其周围牙周组织。采用免疫组化方法,检测局部牙周膜组织中BMP-2的变化。结果:牙周膜成纤维细胞、成骨细胞、成牙骨质细胞及胶原纤维中均可见BMP-2阳性染色。实验对照组与正常组的牙周膜BMP染色相似;静磁场作用后,实验组牙周膜组织中:BMP-2增多。统计学分析表明,在加磁场后的第4、7天,实验组与各对照组间存在显著差异,P<0.05。结论:静磁场可促进牙周膜细胞分泌BMP-2,推测静磁场对牙槽骨的生长具有一定的修复作用。

The effect of static magnetic field on bone morphogenetic protein-2 in periodontal membrane of the rat

PURPOSE: The purpose of this study is to evaluate the effect of static magnetic field on the expression of BMP-2 in the periodontal membrane of rat. METHODS: 35 female rats of wister were randomly divided into three groups, which were the normal group, experimental control group and experimental group. The experimental rat' s cheeks were put into magnet with the intensity of magnetic field being 0.12 tesla. The rats were sacrificed at 2 days, 4 days, 7 days, respectively. Immunohistochemical assays were used to evaluate the changes of BMP-2 expression in the periodontal membrane of the maxillary molars. RESULTS: BMP-2 was mainly found in the plasma of fibroblast, osteoblast, cementocyte, odontoblast separately. There were more BMP-2 in the experimental group treated with static magnetic field. There were significant differences between the experiment group of 4 day and 7 day and the control group. CONCLUSIONS: Static magnetic field stimulated BMP-2 secreted by the cell of periodontal membrane and played an important role in repairing and remodeling of periodontitis.