榄香烯联合热疗诱导乳腺癌MCF-7细胞凋亡及其对细胞周期的影响

目的探讨榄香烯联合热疗诱导人乳腺癌细胞(MCF-7细胞)增殖抑制率,细胞周期各时相改变及凋亡率,以确定联合方案是否具协同效应。方法应用MTT比色法检测MCF-7细胞的增殖抑制率,流式细胞仪检测MCF-7细胞周期各时相变化,电子显微镜观察亚细胞结构改变。结果单纯热疗组,榄香烯组,榄香烯联合热疗组与对照组相比,差异非常显著,P0.01,且MCF-7细胞周期进程发生明显改变,G1期细胞增多,S期细胞减少,G2/M期细胞相对增多,细胞凋亡率升高。电子显微镜结果显示细胞染色质趋边凝集,线粒体肿胀,有空泡形成及凋亡小体改变。结论榄香烯联合热疗可显著提高MCF-7细胞增殖抑制率,抑制MCF-7细胞G1期向S期转化进程,诱导细胞凋亡及亚细胞结构改变。

The influence on apoptosis and life cycle of MCF-7 mammary adenocarcinoma cell inducing by Elemene combined with thermotherapy

Objective To probe the generation inhibition ratio,change of cell life cycle phases and Apoptosis ratio of MCF-7 mammary adenocarcinoma cell inducing by Elemene combined with thermotherapy in order to determine whether it has synergistic effect.Methods MTT was used to detect the generation inhibition ratio of MCF-7 cell,flow cytometer was used to detect the change of life cycle phases and electronmicroscope was used to observe the chang of subcellular structure.Results Simple thermotherapy group,elemene group,Elemene combined with thermotherapy group contrast to control group has significant difference,P0.01.The process of MCF-7 cell life cycle happened to obviously changed,cell in phase G1 was increase,cell in phase S was decrease,cell in phase G2/M was relatively increase,the apoptosis ratio was raised.The electronmicroscope manifested that the cyto-chromatin agglutinated tendering to side,the chondriosome engorged,vacuolation and the change of apoptotic body.Conclusion Elemene combined with thermotherapy can significant enhance the generation inhibition ratio of MCF-7 cell,inhibit the transformation process from phase G1 to phase S of MCF-7 cell and induce apoptosis and the change of subcellular structure.