一株硝化杆菌的筛选及初步鉴定

目的：筛选、分离能够用于治理氮污染的硝化菌。方法：通过富集培养从土壤中筛选自养型硝化菌，在进行形态学和生化的检验之后设计引物，扩增硝化菌的特征性基因norB之后对PCR产物进行测序，并与Genbank上的序列比较同源性。结果：从土壤中筛选到了1株能够氧化亚硝酸盐的细菌，该菌形态小、浅黄色菌落，是杆菌。PCR能扩增出预期大小片段。对其PCR产物直接测序，与Genbank上的序列比较，发现该基因与硝化菌N hamburgensis的norB基因同源性高达99％。结论：可以初步断定该研究所筛选到的细菌属于硝化杆菌。

Preliminary Screen and Identification of One Nitrobacteria

Objective To screen and seperate nitrobacteria that could be used to treat nitrogenous compound pollution.Methods Autotrophic nitrite-oxidizing microorganisms were screened by enrichment culture from soil.After morphological and biological detection,primers were designed to detect the distinctive gene norB of nitrobacteria.The PCR production was se-quenced,and the homology was identified according to the sequence in Genbank.Results One strain of bacterium was screened that could oxidize nitrite.This bacterium was small,light-yellow bacilli.The expected-size DNA fragments could be amplicated by PCR.The sequence of a PCR production was blasted in Genbank and showed99%consistent with norB gene of N hamburgensis.Conclusion It was preliminarily confirmed that the microorganism screened in this study might be nitrobac-terium.