| p53基因和端粒酶活性在良、恶性梗阻性黄疸中的表达 |
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| 引用本文： | 李汛,李玉民,周文策,孟文勃,张磊,何明彦.p53基因和端粒酶活性在良、恶性梗阻性黄疸中的表达[J].兰州医学院学报,2010,36(1):13-16. |
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| 作者姓名： | 李汛 李玉民 周文策 孟文勃 张磊 何明彦 |
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| 作者单位： | 兰州大学第一医院普外二科,甘肃兰州730000 |
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| 基金项目： | 甘肃省自然科学基金(ZS001-A23-075-Y) |
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| 摘 要： | 目的探讨p53基因和端粒酶活性在良、恶性梗阻性黄疸中的表达。方法选取恶性梗阻性黄疸患者64例作为实验组，良性梗阻性黄疸患者60例作为对照组，采用端粒重复序列扩增-聚合酶链式反应-酶联免疫吸附测定法检测端粒酶活性，流式细胞仪检测p53蛋白的阳性表达率。结果恶性和良性梗阻性黄疸组织标本中端粒酶表达阳性率分别为87．50％和3．33％，p53蛋白阳性表达率分别为71．88％和0。结论端粒酶活性和p53蛋白阳性表达与淋巴转移率和肿瘤的分化程度有明显关系。端粒酶和p53基因可以作为胆、胰系统恶性肿瘤诊断的有力标志物，联合检测可以提高恶性梗阻性黄疸的确诊率。
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| 关 键 词： | p53基因 端粒酶 梗阻性黄疸 |
Expression of telomerase and p53 gene in benign and malignant obstructive jaundice |
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| LI Xun,LI Yu-min,ZHOU Wen-ce,MENG Wen-bo,ZHANG Lei,HE Ming-yan.Expression of telomerase and p53 gene in benign and malignant obstructive jaundice[J].Journal of Lanzhou Medical College,2010,36(1):13-16. |
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| Authors: | LI Xun LI Yu-min ZHOU Wen-ce MENG Wen-bo ZHANG Lei HE Ming-yan |
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| Institution: | LI Xun,LI Yu-min,ZHOU Wen-ce,MENG Wen-bo,ZHANG Lei,HE Ming-yan |
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| Abstract: | Objective To investigate the expression of telomerase and p53 gene in biliary-pancreatic system cancer.Methods 64 patients with malignant biliary obstruction were selected as experimental group and 60 patients with benign biliary obstruction as control group.The telomerase activity was tested with telomere repeat amplification protocol-polymerase chain reaction-enzyme linked immunosorbent assay and the expression of p53 was tested by flow cytometry.Results The telomerase activity and p53 positive rate of pa... |
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| Keywords: | p53 gene telomerase obstructive jaundice |
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