前列腺癌p53、bcl-2、bax基因表达与凋亡、增殖的关系

目的:研究前列腺癌组织中细胞增殖与细胞凋亡,及其相关蛋白表达意义。方法:采用原位细胞凋亡标记技术(TUNEL)及免疫组织化学ABC法对36例前列腺癌(Pca)和11例正常前列腺(NP)组织石蜡切片p53、bcl鄄2、bax、PCNA蛋白及细胞凋亡检测。结果:前列腺癌细胞的增殖指数和细胞凋亡指数较NP明显增高,且AI/PI比值较正常组织AI/PI低(P<0.01);随着肿瘤分级的增加,细胞增殖水平明显增加(P<0.01)。p53蛋白表达与前列腺癌细胞增殖水平相关;bcl鄄2蛋白表达与细胞凋亡水平相关(P<0.05);bax基因表达与凋亡无关,但发现它们的bcl鄄2/bax的比值与凋亡有关。表明高bcl鄄2/bax比值多见于低凋亡组,低bcl鄄2/bax比值多见于高凋亡组。结论:细胞增殖与细胞凋亡均参与了前列腺癌的发生、发展。p53基因与细胞增殖水平的调控有关。bcl鄄2和bax在细胞凋亡调节中起到重要作用,由于bcl鄄2蛋白过量表达引起bcl鄄2/bax失平衡,在前列腺癌发生、发展过程中起到重要作用。

Investigation on apoptosis, proliferation and related genes in prostate cancer

Objective: To investigate relationship between proliferation and apo ptosis and the expression of related genes in prostate cancer. Methods: The expr ession of p53, bcl-2, bax and PCNA protein and apoptosis cell were detected by i mmunohistochemical method and in site end labeling method(TUNEL) respectively in tissues of 36 prostate carcinoma cases and 11 cases of normal prostate (NP). Re sults: There was significant difference in both proliferative and apoptotic inde xes between the tumor and normal tissues(P < 0.05), but the rate of AI/PI of the prostate carcinoma were higher than that of the normal prostate (P < 0.01). The increment of proliferative index was positively correlated with pathological gr ade(P < 0.01). The expression of p53 and bcl-2 is separately correlated well wit h proliferative index and apoptosis index. Conclusion: The results suggest that proliferation and apoptosis were both involved in the development of the prostat e cancer.p53 was correlated with the modulation of proliferation respectively; b cl-2 and bax play an important role in apoptosis regulation, the imbalance of bc l-2/bax mainly induced by the undue expression of bcl-2 play an important role i n the development of prostate cancer.