Experiences in implementing uHTS--cutting edge technology meets the real world |
| |
Authors: | Gribbon Philip Schaertl Sabine Wickenden Malcolm Williams Gareth Grimley Rachel Stuhmeier Frank Preckel Hartwig Eggeling Christian Kraemer Joachim Everett Jeremy Keighley Wilma W Sewing Andreas |
| |
Affiliation: | Pfizer Global Research and Development, Lead Discovery Technologies, IPC 580, Ramsgate Road, Sandwich CT13 9NJ, UK. |
| |
Abstract: | Driven by growing corporate compound files, the demands of target biology, and attempts to cut cost, the number of solutions to HTS has spiralled. In quick succession new assay technologies and screening platforms are appearing on the market, with the promise of screening faster than ever in low volume high density formats whilst providing high quality data. Within this world of rapid change, Pfizer has applied cutting edge technology to HTS by introducing screening in 1 microl formats utilising single molecule detection technology. Instead of resource intensive in-house development, Pfizer entered into a collaboration with Evotec OAI / Evotec Technologies and introduced their Mark-II EVOscreen platform. In this article we will outline the benefits of the approach taken at Pfizer, Sandwich, and introduce the Mark-II EVOscreen platform, illustrating the potential but also possible pitfalls of HTS miniaturisation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|