糖尿病患者慢性并发症与外周血单核细胞血红素加氧酶1表达的相关性研究

目的探讨初诊2型糖尿病患者慢性并发症与外周血单核细胞血红素加氧酶1（HO-1）表达的相关性。方法初诊2型糖尿病36例分为并发症组和无并发症组，正常对照组（NC）10名，比较各组空腹血糖（FPG）、血清丙二醛（MDA）水平、外周血单核细胞活性氧（ROS）产量及HO-1表达水平，并分析其相关性。结果糖尿病各组的FPG、MDA、ROS和HO-1水平均显著高于NC组（P〈0．01），有并发症组的上述各指标均显著高于无并发症组（P〈0．05）。HO-1 mRNA分别与HO-1蛋白、MDA、FPG水平显著正相关，HO-1蛋白水平与ROS、MDA显著正相关，MDA与ROS显著正相关（P〈0．05）。结论初诊2型糖尿病合并慢性并发症患者的高血糖引起氧化应激并诱导HO-1适应性和代偿性增高，将来有望通过适当诱导HO-1高表达以拮抗糖尿病氧化应激。

Relationship between diabetic chronic complications and expression of HO-1 in peripheral blood mononuclear cells in newly diagnosed type 2 diabetic patients

Objective To investigate the relationship between diabetic chronic complications and expression of HO-1 in peripheral blood mononuclear cells （PBMC） in newly diagnosted type 2 diabetie patients. Methods There were two groups of newly diagnosed type 2 diabetic patients, chronic diabetic complication group and non-complication group. Fasting plasma glucose （FPG）, HbA1c, serum MDA, ROS output and expression of HO-1 mRNA and protein in peripheral blood monoeytes were determined. Also health volunteers were used as control group. Results Control group had significantly lower levels of FPG, MDA, ROS output, HO-1 mRNA and protein than diabetic group. Complication group had significantly higher levels of FPG （P〈0. 05）, HbA1c （P〈0.05）, ROS output （P〈0.01）, serum MDA （P〈0. 01）, and expressions of HO-1 mRNA （P〈0. 05） and protein （P〈0. 05） when compared with non- complication group. There was positive correlation between the expression of HO-1 mRNA and levels of FPG, MDA and HO-1 protein （P〈0.05）. Also there were positive correlation between the expression of HO-1 protein and ROS output, serum MDA （P〈0. 05）. And MDA was correlated with ROS significantly （P〈 0. 05）. Conclusions There are significant oxidative stress in newly diagnosed type 2 diabetic patients with chronic complications. HO-1 may be one of the most important protective mechanisms against oxidative stress induced by diabetes mellitus, and high expression of HO-1 may prevent or prolong the development of diabetic chronic complications.