口腔洁含漱液对脾胃伏火型复发性口腔溃疡患者口腔菌群、血清炎症因子和T 细胞亚群水平的影响

目的 探讨口腔洁含漱液对脾胃伏火型复发性口腔溃疡患者口腔菌群、血清炎症因子和T细胞亚群水平的影响。方法 选取2022年4月至2023年3月成都中医药大学附属医院口腔科和四川大学华西口腔医院口腔黏膜科收治的脾胃伏火型复发性口腔溃疡患者64例为研究对象，按照随机数字表法分为试验组(32例)和对照组(32例)，对照组患者给予醋酸曲安奈德乳膏治疗，试验组在对照组基础上联合口腔洁含漱液含漱、吞服，每日3次，持续治疗7 d。观察两组患者临床疗效，评价两组治疗前后，以及治疗后组间的中医证候积分、疼痛指数、疼痛持续时间和溃疡愈合时间；实时荧光定量PCR法分析口腔菌群(链球菌、韦荣氏菌、奈瑟氏菌)DNA浓度；ELISA法检测血清炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6、IL-8]含量；流式细胞术测定血清T细胞亚群水平[T淋巴细胞(CD3⁺)、辅助性T淋巴细胞(CD4⁺)、CD4⁺/细胞毒性T淋巴细胞(CD8⁺)]。同时记录治疗期间不良反应，比较6个月后复发情况。结果 试验组临床疗效、疼痛抑制效果...

Effect of Kouqiangjie Gargle on Oral Microflora， Serum Inflammatory Factors and T Cell SubgroupLevels in Patients with Recurrent Oral Ulcer Caused by Fire Hidden in the Spleen and Stomach

To explore the effects of Kouqiangjie （KQJ） Gargle on the oral microflora，serum inflammatoryfactors and T cell subgroup levels in patients with recurrent oral ulcer （ROU） caused by fire hidden in the spleenand stomach. Methods From April 2022 to March 2023，64 patients with ROU caused by fire hidden in the spleenand stomach，who were treated in the Department of Stomatology，the Hospital of Chengdu University of TraditionalChinese Medicine （TCM） and Department of Oral Mucosa， West China Hospital of Stomatology， SichuanUniversity， were enrolled as the research object. All patients were randomly divided into trial group （n=32） andcontrol group （n=32） according to random number table method. Patients in the control group were treated withTriamcinolone Acetonide Acetate Cream，while patients in the trial group received the same treatment of the controlgroup combined with KQJ Gargle rinsing and swallowing， 3 times daily for 1 week. Clinical efficacy of the twogroups were compared. The traditional Chinese medicine （TCM） syndrome scores， pain index， pain duration andulcer healing time were recorded. The DNA concentrations of oral microflora （Streptococcus， Veillonella andNeisseria） were detected by real-time quantitative polymerase chain reaction （RT-qPCR）， enzyme-linkedimmunosorbent assay （ELISA） was used to determine the levels of serum inflammatory factors [tumor necrosisfactor-α （TNF-α），interleukin-6 （IL-6） and IL-8] ，and flow cytometry was applied to examine the level of Tcell subsets ［T lymphocytes （CD3+），helper T lymphocytes （CD4+），CD4+/cytotoxic T lymphocytes （CD8+）］. Theadverse reactions during treatment were observed and recorded， and the recurrence rate after 6 months were alsocompared. Results The clinical efficacy，pain inhibitory effect and ulcer healing rate of trial group were higher thanthose in control group （P＜0.05）. After treatment，the DNA concentrations of Streptococcus，and Veillonella weresignificantly higher than those before treatment in both groups. Moreover，the trial group was higher than the controlgroup，with statistical significance （P＜0.05）. The levels of serum inflammatory factors （TNF-α，IL-6 and IL-8）in two groups after treatment were diminished when compared to prior-treatment， while the levels of CD3+， CD4+and CD4+/CD8+ were raised， and the improvement degree was greater in the trial group than in the control group.The difference was statistically significant （P＜0.05）. The recurrence rate in the trial group was significantly lowerthan that in the control group （P＜0.05）. Conclusion KQJ Gargle can significantly improve the therapeutic effect，ameliorate the compositions of oral microflora， inhibit serum inflammatory response levels， enhance the cellularimmune functions， and reduce recurrence rate in the treatment of patients with ROU caused by fire hidden in thespleen and stomach，so it is worthy of clinical application.