血性羊水细胞培养方法的改良与应用

摘要:目的比较血性 羊水不同培养方法的成功率。方法收集 2021年1月至2022年12月安徽省妇幼保健院行染色体检查的血性羊水标本31例,31例均采用两线培养法:1线载玻片原位培养盒法(简称原位培养法),1线塑料培养瓶和原位培养盒联合培养法(简称联合培养法),收获培养细胞并行吉姆萨染色,比较两种培养方法的成功率和有效染色体核型数量。结果原位培养法31例,21例培养成功,成功率67.7%;联合培养法31例均培养成功,成功率100%;联合培养法成功率高于原位培养法(P<0.05)。31例血性羊水中,3例新鲜出血,原位培养法平均有效核型数为8,联合培养法平均有效核型数为32;28例陈旧性出血,原位培养法平均有效核型数为13,联合培养法平均有效核型数为53。联合培养法的有效核型数高于原位培养法(P<0.05)。 结论联合培养法适用于血性羊水标本的培养,值得推广应用。

Improvement and application of culture method for hemorrhagic amniotic fluid cells

Abstract: Objective To compare the success rates of different culture methods for hemorrhagic amniotic fluid. Methods Thirty-onehemorrhagic amniotic fluid samples from pregnant women who were subjected to chromosomal examination at Anhui Maternal and Child Health Hospital were collected from January 2021 to December 2022. Two culture methods, the slide in situ culture box method ( referred to as the slide method) and the plastic bottle culture combined with slide in situ culture box method ( referred to as the combined culture method),were used for cell cultivation. All the cells were harvested and stained with Giemsa staining, then the number of eligible karyotype was counted and the success rates were compared between the two methods. Results Among the 31 cases of the slide method, 21 were successfully cultured with a success rate of 67.7%. For the combined culture method, all the 31 cases were successfully cultured with a success rate of 100%. The success rate of the combined culture method was significantly higher than that of the slide method ( P<0.05). Of 31 bloody amniotic fluid samples,three cases were fresh bleeding, and an average number of eligible karyotype was 8 in the slide method and 32 in the combined culture method. Twenty-eight cases were old bleeding, and an average number of eligible karyotype was 13 in the slide method and 53 in the combined culture method. The number of eligible karyotype in the combined culture method was significantly higher than that of the slide method ( P<0.05). Conclusion The combined culture method is suitable for the cultivation of hemorrhagic amniotic fluid samples,and should be worthy of promotion in the clinics.