UPLC-Q-TOF-MS/MS 技术分析参苓白术散化学成分及入血成分

目的 研究参苓白术散的化学成分及大鼠灌胃给药参苓白术散后大鼠血浆中的化学成分。方法 采用超高效液相色谱-四极杆/飞行时间质谱（UPLC-Q-TOF-MS/MS）技术，通过Peak View 1.2和Chem Draw软件，根据保留时间、相对分子质量及二级离子碎片，结合对照品及文献数据，对参苓白术散的化学成分及入血成分进行鉴别。结果 从参苓白术散中鉴定出104个化学成分，主要为黄酮类和有机酸类成分；在灌胃后大鼠血浆中鉴定出27个入血成分，包括11个原型成分和16个代谢产物，主要的代谢途径为Ⅱ相代谢途径。结论 该研究通过UPLC-Q-TOF-MS/MS技术快速分析了参苓白术散的化学成分及入血成分，可为阐明参苓白术散的药效物质基础及其作用机制提供参考。

Analysis on Chemical Composition and Constituents Absorbed in the Blood of Shenling Baizhu San byUPLC-Q-TOF-MS/MS

To study the chemical composition of Shenling Baizhu San and the components in rat plasmaafter intragastric administration of Shenling Baizhu San. Methods UPLC-Q-TOF-MS/MS was used for the analysis.According to the retention time， relative molecular weight and secondary ion fragments， as well as referencesubstance and literature data，the chemical composition and constituents absorbed in the blood of Shenling BaizhuSan were identified by using Peak View 1.2 and Chem Draw software. Results A total of 104 chemical componentswere identified from Shenling Baizhu San，mainly contained flavonoids and organic acids. There were 27 componentsin rat plasma found after intragastric administration of Shenling Baizhu San，including of 11 prototype componentsand 16 metabolites. The main metabolic pathway was phase-Ⅱ metabolic pathway. Conclusion In this study，thechemical composition and constituents absorbed in the blood of Shenling Baizhu San were rapidly analyzed by UPLCQ-TOF-MS/MS technology，which provided a reference for further elucidating the pharmacodynamic substance basisand mechanism of Shenling Baizhu San