黄芩汤治疗蓖麻油致小鼠腹泻的作用及机制研究

目的 研究黄芩汤对蓖麻油致小鼠腹泻模型的作用及作用机制。方法 昆明小鼠随机分为对照组、模型组、洛哌丁胺（5 mg/kg）组和黄芩汤高、中、低剂量（20、10、5 g/kg）组，每组10只。连续3 d ig给药，末次给药30 min后ig 0.5 mL蓖麻油制备腹泻模型。测定腹泻评分和腹泻指数，采用qRT-PCR检测肝脏中α-1-酸性糖蛋白（alpha 1-acid glycoprotein,AGP）、C反应蛋白（C-reactive protein,CRP）、转铁蛋白（transferrin,TRF）、白蛋白（albumin,ALB）和小肠中水通道蛋白3(aquaporin 3,AQP3)、AQP4、Na/H离子交换器2(Na/H exchanger 2,NHE2)、NHE3、NHE8 m RNA表达；采用免疫组化法和Western blotting检测小肠中AQP3和NHE8蛋白表达；收集肠道内容物，进行16S rRNA测序。结果 黄芩汤显著降低小鼠的腹泻评分和腹泻指数（P<0.05），下调肝脏AGP、CRP的m RNA表达（P<0.05），下调小肠AQP3、NHE8的m...

Effect and mechanism of Huangqin Decoction in treatment of castor oil-induced diarrhea mice

Objective To study the effect and mechanism of Huangqin Decoction (黄芩汤) on castor oil-induced diarrhea model in mice. Methods Kunming mice were randomly divided into control group, model group, loperamide (5 mg/kg) group, and Huangqin Decoction high-, medium-, and low-dose (20, 10, 5 g/kg) groups, with 10 mice in each group. Drugs were administered intragastrically for three consecutive days, a diarrhea model was prepared by administering 0.5 mL castor oil at 30 min after the last administration. Diarrhea score and diarrhea index were measured, and qRT-PCR was used to detect mRNA expressions of alpha 1-acid glycoprotein (AGP), C-reactive protein (CRP), transferrin (TRF), albumin (ALB) in liver and aquaporin 3 (AQP3), AQP4, Na/H exchanger 2 (NHE2), NHE3 and NHE8 in small intestine; Immunohistochemistry and Western blotting were used to detect the expressions of AQP3 and NHE8 proteins in small intestine; Intestinal contents were collected for 16S rRNA sequencing. Results Huangqin Decoction significantly reduced the diarrhea score and diarrhea index of mice (P < 0.05), downregulated the mRNA expressions of AGP and CRP in liver (P < 0.05), downregulated the mRNA and protein expressions of AQP3 and NHE8 in small intestine (P < 0.05), reduced the abundance of Bifidobacterium, Faecalibacterium and Ruminococcaceae_UCG-014, increased the abundance of Jeotgallicoccus and Candidatus_Arthromitus, decreased the ratio of Firmicutes to Bacteroidetes. Conclusion Huangqin Decoction may downregulate the expressions of intestinal epithelial transport proteins and acute phase proteins, alter gut microbiota, and thus alleviate diarrhea caused by castor oil.