体外培养人成纤维细胞转化生长因子-β1自分泌规律的研究

目的　探讨体外培养人成纤维细胞转化生长因子 -β1 (TGF-β1 )的自分泌规律。方法　应用健康人包皮环切术所得包皮 ,通过体外成纤维细胞传代培养共 12代 ,运用 EL ISA法 ,分别检测不同代次成纤维细胞 TGF-β1 浓度 ,并测定第 6代成纤维细胞在不同时间培养上清液中 TGF-β1 的浓度。结果　自第 3代开始 ,成纤维细胞培养上清液可检测到 TGF-β1 ,并逐渐升高 ,至第 6代达分泌高峰 (45 0 ng/ L ) ,第 11、12代不能测到 ;而第 6代成纤维细胞 ,培养上清液中 TGF-β1 含量以第 5天的检测值最高 (6 80 ng/ L ) ,是第 1天检测值 (2 80 ng/ L )的 2 .5倍。结论　成纤维细胞 TGF-β1 自分泌能力 ,是由弱到强再逐渐减弱的过程 ,有自我调节能力

AUTOCRINE REGULATION FOR TRANSFORMING GROWTH FACTOR-β1 BY FIBROBLAST IN VITRO

OBJECTIVE: To explore the autocrine regulation for the transforming growth factor-beta 1(TGF-beta 1) by the fibroblasts in vitro. METHODS: Fibroblasts were cultured in vitro which isolated from the normal prepuce by circumcision. TGF-beta 1 concentration were determined with ELISA for the different passages and the different time at the same passage. RESULTS: The TGF-beta 1 concentration was different for the distinct passage of fibroblasts and achieved the peak (450 ng/L) at the sixth passage. Among the same fibroblasts, the TGF-beta 1 concentration was different in the various days and passage of the summit (680 ng/L) on the fifth day, which was 2.5 times as high as that of the first day. CONCLUSION: The fibroblasts possess the ability of autocrine of the TGF-beta 1 and have some regularity. It will provide the theory basis for the research about TGF-beta 1 regulation mechanism and the medical application about salvia miltiorrhiza.