乙型肝炎患者外周血B淋巴细胞CD5 mRNA表达水平的研究

目的 建立CD5 mRNA的逆转录聚合酶链反应（RTP－PCR)测定法，探讨外周血B淋巴细胞CD5 mRNA水平与乙型肝炎慢性化和严重化的关系。方法 运用RT－PCR技术，建立了B淋巴细胞中CD5基因mRNA表达水平的半定量测定方法，对急、慢性乙型肝炎和肝炎后肝硬化患者进行了研究。结果 急性乙型肝炎患者组外周血B淋巴细胞中CD5 mRNA的水平与正常对照组无显著性差别，其余各乙型肝炎患者组均显著高于对照组。且肝炎后肝硬化组＞慢性乙型肝炎组＞急性肝炎组（P＜0．01）。结论 外周血B淋巴细胞CD5 mRNA水平与乙型肝炎的发病和慢性化发展密切相关。

CD5 mRNA level in peripheral blood B lympocytes of patients with hepatitis B

Objectives To establish the semi-quantitive RT-PCR method for detecting CD5 mRNA and investigate the correlation between CD5 mRNA level in B lymphocytes of peripheral blood from hepatitis B patients and persistence and severity of the disease.Methods The semi-quantitative RT-PCR method was established to detect CD5 mRNA in B lymphocytes from hepatitis B patients.The patients with acute,chronic hepatitis B and posthepatitis B cirrhosis were investigated.Results There was no significant diffcrence in CD5 mRNA level B lymphocytes between acute hepatitis B patients and the controls,while the CD5 mRNA levels in the patients with chronic hepatitis B,chronic severe hepatitis B and posthepatitis B cirrhosis were significantly higher than that of controls(P<0.01).Conclusions CD5 mRNA level in B lymphocytes of peripheral blood from hepatitis B patients is closely related with persistant process of the disease.