siRNA特异性靶向沉默Livin基因对人乳腺癌MCF－7多药耐药细胞耐药性的研究

目的研究siRNA特异性靶向沉默Livin基因对人乳腺癌MCF－7多药耐药细胞耐药性的影响。 方法采用ADM低浓度梯度递增结合大剂量间歇诱导方法建立5－FU诱导的多药耐药细胞系，采用ATP生物荧光肿瘤体外药物检测技术(ATP－TCA)法检测细胞耐药性，采用免疫组织化学方法检测Livin蛋白在MCF－7耐药细胞株系中的表达情况，采用Livin SiRNA联合表柔吡星、5－氟尿嘧啶、多西他赛、健择诱导乳腺癌MDR细胞的凋亡。采用SPSS 20.0处理数据，耐药情况、细胞的凋亡情况用( ±s)表示，采用t检验，当P<0.05时差异具有统计学意义。 结果结果显示，MCF－7多药耐药细胞存在多药耐药问题；联合组MCF－7增敏(19.48±12.00) μM、MCF－7/MDR增敏(35.62±2.37) μM，与转染组与加药组对比，数据差异具有统计学意义(P<0.05)。 结论siRNA特异性靶向沉默Livin基因可增强人乳腺癌MCF－7多药耐药细胞的敏感性，提高细胞凋亡率。

Study of siRNA specific targeted silencing of Livin gene on multidrug resistance in human breast cancer MCF-7 cells

ObjectiveTo investigate the effect of siRNA targeted silencing of Livin gene on multidrug resistance of human breast cancer MCF-7 cells. MethodsADM low concentration gradient induced method combined with high-dose intermittent was used to establish multidrug resistance cell line 5-FU induced by ATP in vitro, bioluminescence tumor drug detection (ATP-TCA) method was used for the detection of drug resistant cells, immunohistochemical method was used to detect the expression of Livin protein in MCF-7 resistant cell lines, using Livin combined with SiRNA epirubicin, fluorouracil, docetaxel , gemzar, apoptosis was induced in breast cancer MDR cells. ResultsThe results showed that there was a multidrug resistance problem in MCF-7 multidrug resistant cells, and the combination group MCF-7 was sensitized (19.48+ 12) and MCF-7/MDR was sensitized (35.62+ 2.37) mu M, and the data were statistically significant (P<0.05) compared with those in the transfected group and the addition group. ConclusionsiRNA specific targeted silencing of Livin gene can enhance the sensitivity of human breast cancer MCF-7 multidrug resistance cells and increase the rate of apoptosis.