电纺PLLA/PVP纳米纤维膜材料用于血管组织工程的前期研究

目的:研究静电纺丝在血管组织工程中的应用。方法:利用静电纺丝技术制备不同质量分数的聚乳酸(PLLA)/聚乙烯吡咯烷酮(PVP)电纺丝膜,并通过差示扫描量热分析(DSC)、扫描电子显微镜(SEM)观察、接触角测试(CA)和力学性能分析进行材料学表征。在不同的电纺丝膜上种植血管平滑肌细胞(VSMCs),并分别在2、4、6 d取样进行SEM、激光共聚焦显微镜(LCMS)观察,以检测VSMCs在材料上铺展的形貌和分布情况。经MTT测试,以了解材料对VSMCs增殖的影响,用血小板黏附实验测试材料的血液相容性。结果:混入PVP的PLLA电纺丝膜具有良好的表面结构,亲水性显著提高,且随着PVP含量的增多PLLA电纺丝膜的亲水性增高,但对力学性能影响不明显。细胞实验结果表明:混有PVP的PLLA电纺丝膜利于VSMCs的黏附生长,具有良好的细胞相容性和血液相容性。结论:成功地将PVP和PLLA进行了静电纺丝,该材料具有良好的细胞相容性和血液相容性,其在血管组织工程应用中具有广阔的前景。

Preliminary study for vascular tissue engineering by electrospinning PLLA/PVP nanofiber films

Objective: To investigate the application of electrospinning in vascular tissue engineering.Methods: PLLA and PVP with different ratios were prepared by electrospinning,and the properties of the composite nanofiber films were investigated by differential scanning calorimetry(DSC),scanning electron microscopy(SEM),contact angle measurement(CA) and Mechanical Test.Vascular smooth muscle cells(VSMCs) were used to test the cytocompatibility.Cell morphology and cell proliferation were measured by SEM,laser scanning confocal microscopy(LCMS) and MTT assay after 2,4,6 days of culture.In addition,adhesion of platelet on the films was observed by SEM for testing the blood compatibility.Results: The SEM images showed that the morphology of the microfiber films was mainly affected by the weight ratios of PLLA/PVP.The DSC results demonstrated that PLLA and PVP mixed uniformly.And the hydrophilicity of the films measured increased along with the decrease of the PLLA/PVP ratio.The mechnical test indicated that the covered PLLA/PVP fiber films with different weight ratios had no difference.The cell experiments showed that the composite films had better cytocompatibility and blood compatibility.Conclusion: The electrospinning nanofiber composite films of PLLA and PVP would have potential use as the scaffolds for vascular tissue engineering.