人工培养冬菇菌丝体中抗血栓形成成分的分离和生物学性质

从人工培养冬菇菌丝体中用阳离子交换纤维素层析分离,获得一种具有抗血栓形成作用的蛋白质,分子量为1.75×10~4u,等电点为4.3,给大鼠iv10~(-6)mol1/kg,能完全预防体外血栓形成,IC_(50)为4.4×10~(-7)mol/kg。无论是体内或体外,都具有对抗胶原引起的血小板聚集作用,体外全对抗浓度为7.5×10~(-5)mol/L,其IC_(50)为4×10~(-5)1/L,体内剂量1.25×10~(-5)mol/kg(iv)时,血小板聚集抑制率可达60%,抗血凝的最低浓度为3 ×10~(-6)mol/L。

ISOLATION AND PHARMACOLOGICAL ACTIVITIES OF AN ANTITHROMBUS PROTEIN FROM FLAMMULINA VELUTIPES

A protein designated as Flammulina Velutipes (FV) protein was isolated from the cultured mycelium of FV and purified to homogeneity by CM cellulose CM 52 and gel filtration chromatography. The molecular weight of FV protein were found to be 1.75×10~4u with pI 4.3. Amino acid analysis of FV protein indicated the presence of 14 kinds of amino acids, especially rich in Glu, Asp and Thr. Potent inhibitory effect of FV protein on thrombus formation, in the blood was observed in vitro when rats were given intravenously. The length of thrombus was shortened and the dry weight of the thrombus decreased significantly at the dose of 7.5×10~(-7) mol/kg. Thrombus formation was completely inhibited at the dose of 10~()-8 mol/kg. The IC_(50) was found to be 4.4×10~(-7) mol/kg. FV protein was shown to inhibit collegen induced aggregation of rat platelets both in vitro and in vivo. Its concentration for complete inhibition in vitro was 7.5×10~(-5) mol/L. The IC_(50) was found to be 4×10~(-5)mol/ L. However, FV protein dose of 1.25×10~(-5) mol/kg was required for 60% inhibition in vivo