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| 人骨髓间质干细胞体外培养诱导成为心肌样细胞的实验研究 | |
| 引用本文： | 胡波,周中新,董红燕,张中明.人骨髓间质干细胞体外培养诱导成为心肌样细胞的实验研究[J].徐州医学院学报,2004,24(3):200-202. |
| 作者姓名： | 胡波 周中新 董红燕 张中明 |
| 作者单位： | 1. 徐州医学院附属医院胸心外科,江苏,徐州,221002 2. 徐州医学院第二附属医院胸心外科,江苏,徐州,221006 3. 徐州医学院神经生物学研究中心,江苏,徐州,221002 |
| 基金项目： | 徐州医学院附属医院科研课题资助项目 (2 0 0 2 ) |
| 摘 要： | 目的 研究人骨髓间质干细胞的体外培养及向心肌样细胞转化的条件。方法 取成人髂骨骨髓，分离出骨髓间质干细胞进行培养、传代。观察在培养液中添加诱导剂5-氮胞苷(5-aza)条件下骨髓间质干细胞的生长和分化。结果 成人骨髓间质干细胞贴壁呈集落生长，有成纤维细胞样外观。5-aza诱导人骨髓间质干细胞转化为心肌样细胞。结论 人骨髓间质干细胞能够在体外被诱导分化为心肌样细胞，是心肌组织工程良好的细胞来源。 |
| 关 键 词： | 骨髓间质干细胞 体外培养 心肌样细胞 实验 细胞转化 |
| 文章编号： | 1000-2065(2004)03-0200-03 |
Myocardial cells can be generated from human bone mesenchymal stem cells in vitro |
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| HU Bo,ZHOU Zhong-xin,DONG Hong-yan,et al.Myocardial cells can be generated from human bone mesenchymal stem cells in vitro[J].Acta Academiae Medicinae Xuzhou,2004,24(3):200-202. | |
| Authors: | HU Bo ZHOU Zhong-xin DONG Hong-yan |
| Abstract: | Objective To investigate the growth and myocardial differentiation of human bone mesenchymal stem cells (BMSCs) in vitro. Methods The human BMSCs were taken from the ilium of a volunteer were cultured. 5-azacytidine (5-aza) for myocardial induction was added to the culture medium, the proliferation and differentiation of cells were noticed. Results The BMSCs were found grew into colonies with the appearance of fibroblastic cells and differentiate into myocardial cells. Conclusion Myocardial cells can be generated from human BMSCs in vitro,and the human BMSCs may be one of the adequate resources of donor cells for myocardial engineering. |
| Keywords: | bone mesenchymal stem cells cell culture induction |
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