| Affiliation: | 1.Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim,Heidelberg University, German Red Cross Blood Service Baden-Württemberg- Hessen,Mannheim,Germany;2.Cellex GmbH,Dresden,Germany;3.Medical Department III (Hematology, Oncology),Charité Campus Benjamin Franklin,Berlin,Germany;4.Medical Department III (Hematology, Oncology),University Medical Centre Mannheim, Heidelberg University,Heidelberg,Germany |
| Abstract: | BackgroundCCR5-delta32 heterozygous individuals are susceptible to HIV-1. However, it is not clear if there is a relevant protective effect against transmission and a beneficial effect in terms of HIV progression which cannot be attributed to CCR5 surface density alone. Therefore we investigated HIV-1 dependency factors (HDF) which might be differently regulated in CCR5 wild type (WT) and CCR5-delta32 heterozygous individuals.MethodsWe examined CD34+ hematopoietic progenitor cells derived from bone marrow samples from 19 healthy volunteers, 12 individuals with CCR5 WT and 7 with heterozygous CCR5-delta32 deletion. Samples were analyzed using a global gene expression oligonucleotide microarray (HG-U133plus 2.0, Affymetrix Inc.).ResultsA total of 205 genes were found with altered expression (3fold difference, present call rate of 75%, p? ConclusionThe CCR5-delta32 deletion is associated with other HDFs in HIV-1 pathogenesis as a possible explanation for beneficial effects regarding the deletion leading to a variant expression profile in heterozygous carriers of this mutation. |
