| 红芪多糖3促进小鼠胸腺细胞增殖的差异蛋白质点分析 |
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| 引用本文： | 韦艳霞,卫东锋,赵春燕,程卫东,张李峰,苏刚. 红芪多糖3促进小鼠胸腺细胞增殖的差异蛋白质点分析[J]. 中药药理与临床, 2011, 0(6): 42-46 |
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| 作者姓名： | 韦艳霞 卫东锋 赵春燕 程卫东 张李峰 苏刚 |
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| 作者单位： | 兰州大学基础医学院;兰州大学药学院; |
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| 基金项目： | 中国博士后科学基金资助(20090450017);中国博士后科学基金特别资助(201003073); 教育部高等学校博士点基金项目(200807330006) |
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| 摘 要： | 目的:寻找红芪多糖3(HPS-3)作用后小鼠胸腺组织差异表达的蛋白质点,探讨HPS-3提高免疫力的分子生物学水平机制。方法:正常小鼠给予不同剂量的HPS-3,测定胸腺、脾脏指数及T淋巴细胞增殖能力,并运用双向凝胶电泳技术对胸腺组织蛋白进行分离,银染显色、Image Scanner获取凝胶图谱后,通过PD Quest软件对其进行分析、寻找差异蛋白质点。结果:HPS-3(50mg/kg)对小鼠胸腺指数、脾脏指数及T淋巴细胞增殖能力无显著影响,而100 mg/kg HPS-3能显著增加小鼠胸腺、脾脏指数,增强T淋巴细胞增殖反应。实验所得2-DE图谱经PD Quest软件分析得出以下结果:空白组分离得到1129个蛋白点,HPS-3组得到1167个蛋白质点,仅在空白组出现的蛋白质点有316个,仅在HPS-3组出现的蛋白点有354个,两组共有的蛋白点中表达量差异两倍以上的有192个。结论:HPS-3能促进胸腺细胞增殖,显著影响小鼠胸腺细胞蛋白质表达。
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| 关 键 词： | 红芪多糖 胸腺 双向电泳 差异点 |
Differential protein spots analysis of mouse thymus lymphoid cells proliferation promoted with third polysaccharides from Radix Hedysari |
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| Wei Yanxia,Wei Dongfeng,Zhao Chunyan,Cheng Weidong,Zhang Lifeng,Su Gang. Differential protein spots analysis of mouse thymus lymphoid cells proliferation promoted with third polysaccharides from Radix Hedysari[J]. Pharmacology and Clinics of Chinese Materia Medica, 2011, 0(6): 42-46 |
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| Authors: | Wei Yanxia Wei Dongfeng Zhao Chunyan Cheng Weidong Zhang Lifeng Su Gang |
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| Affiliation: | Wei Yanxia1,Wei Dongfeng1,Zhao Chunyan2,Cheng Weidong1,Zhang Lifeng1,Su Gang1(1School of Basic Medicine,Lanzhou University,Lanzhou 730000,2 School of Pharmacy,Lanzhou 730000) |
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| Abstract: | Objective:To find out the differential protein spots expression in mouse thymus organizations treated with the third polysaccharides from Radix Hedysari,discuss the molecular biology mechanism of enhancing the immunity with HPS-3.Methods:HPS-3 was given into the mice at different doses,then detected the thymus gland index,spleen index and proliferation index of T lymphocyte.Using two-dimensional gel electrophoresis(2-DE) to separate the protein of mouse thymus organizations,then the gels were stained using ... |
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