| 姜黄素对Hepa1-6肝癌细胞凋亡及ROS影响 |
| |
| 引用本文： | 杜琴,邓珊,沈克平,胡兵.姜黄素对Hepa1-6肝癌细胞凋亡及ROS影响[J].中药药理与临床,2011(6):28-31. |
| |
| 作者姓名： | 杜琴 邓珊 沈克平 胡兵 |
| |
| 作者单位： | 上海中医药大学附属龙华医院肿瘤科中医肿瘤研究所; |
| |
| 基金项目： | 上海市基础研究重点项目(09JC1413600); 龙华医院国家中医临床研究基地龙医团队、龙医学者项目(LYTD-04) |
| |
| 摘 要： | 目的:观察姜黄素对小鼠肝癌Hepa 1-6细胞凋亡及活性氧(Reactive oxygen species,ROS)水平的影响。方法:不同浓度姜黄素处理Hepa 1-6肝癌细胞,MTT比色法检测细胞增殖,Hoechst 33258染色检测细胞凋亡形态变化,流式细胞术检测细胞凋亡,Western Blot法检测活化型caspase-3,2-7-二氯氢化荧光素乙二脂(DCFH-DA)染色法结合荧光酶标仪检测姜黄素对胞内ROS产生的影响。结果:2～40μmol/L姜黄素作用24 h、48 h均可抑制肝癌Hepa 1-6细胞增殖,并呈现一定的时间与剂量依赖性。5～20μmol/L姜黄素作用48 h后Hepa 1-6细胞呈现凋亡形态变化,细胞凋亡率呈一定程度剂量依赖性,同时可检测到活化型caspase-3、以及ROS产生。结论:姜黄素可以抑制Hepa 1-6细胞增殖,促使Hepa 1-6细胞凋亡,可能与活化caspase-3及提高细胞内ROS水平相关。
|
| 关 键 词： | 姜黄素 肝癌 细胞凋亡 caspase-3 ROS |
Effects of curcumin on ROS production and apoptosis in murine hepatocarcinoma Hepa 1-6 cells |
| |
| Du Qin,Deng Shan,Shen Keping,Hu Bing.Effects of curcumin on ROS production and apoptosis in murine hepatocarcinoma Hepa 1-6 cells[J].Pharmacology and Clinics of Chinese Materia Medica,2011(6):28-31. |
| |
| Authors: | Du Qin Deng Shan Shen Keping Hu Bing |
| |
| Institution: | Du Qin,Deng Shan,Shen Keping,Hu Bing(Department of Oncology and Institute of Traditional Chinese Medicine in Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 20032) |
| |
| Abstract: | Objective:To observe the effects of curcumin on apoptosis and reactive oxygen species(ROS) production in murine hepatocarcinoma Hepa1-6 cells in vitro.Methods:Hepa1-6 cells were treated with different dose of curcumin.Cell proliferation was detected with MTT assay.Apoptotic morphology was visualized by hoechst 33258 staining.Cell apoptosis was detected by flow cytometry analysis.Caspase-3 was detected by western blot.Intracellular ROS production was detected by 2',7'-dichlorofluorescin diacetate(DCFH-DA) st... |
| |
| Keywords: | curcumin hepatocarcinoma apoptosis caspases-3 reactive oxygen species |
| 本文献已被 CNKI 等数据库收录！ |
|
