| 蝎毒纤溶活性肽对低糖低氧/复氧损伤大鼠皮层神经元的保护作用 |
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| 引用本文： | 王巧云,吴峰阶,杨静.蝎毒纤溶活性肽对低糖低氧/复氧损伤大鼠皮层神经元的保护作用[J].中药药理与临床,2011(4):20-23. |
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| 作者姓名： | 王巧云 吴峰阶 杨静 |
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| 作者单位： | 滨州医学院基础学院;滨州医学院临床学院; |
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| 基金项目： | 2009年山东省高等学校优秀青年教师国内访问学者资助 |
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| 摘 要： | 目的:研究蝎毒纤溶活性肽(SVFAPs)对大鼠神经元低糖低氧/复氧损伤的影响,探讨药物发挥神经保护作用的可能机制。方法:建立原代培养的大鼠皮层神经元的低糖低氧/复氧(OGD/R)模型,采用四唑蓝(MTT)法和乳酸脱氢酶(LDH)释放法检测细胞活力;检测细胞上清液中丙二醛(MDA)和一氧化氮(NO)含量、超氧化物歧化酶(SOD)活性;Hoechst染色和DNA片段法检测细胞凋亡;W estern b lot法检测PARP蛋白的表达。结果:经OGD/R损伤后细胞活力明显下降,细胞上清液中MDA、NO升高,SOD降低;出现凋亡典型的形态学特征,PARP蛋白表达下降;蝎毒纤溶活性肽(2～8mg/L)可不同程度提高低糖低氧/复氧后神经元的活力,降低细胞上清液MDA和NO含量,提高SOD活性。改善凋亡相关的细胞核形态学改变,并且减少凋亡特征性DNA片段化的发生,降低细胞凋亡率,保持PARP的完整性。结论:蝎毒纤溶活性肽对低糖低氧/复氧损伤的神经元具有保护作用,其机制可能与抗氧化和减轻细胞凋亡有关。
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| 关 键 词： | 蝎毒纤溶活性肽 皮层神经细胞 低糖低氧/复氧 氧化 凋亡 |
Protective effect of scorpion venom fibrinolytic active peptideson anoxia/reoxygenation injury of cortex neurons |
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| Wang Qiaoyun,Wu Fengjie,Yang Jing.Protective effect of scorpion venom fibrinolytic active peptideson anoxia/reoxygenation injury of cortex neurons[J].Pharmacology and Clinics of Chinese Materia Medica,2011(4):20-23. |
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| Authors: | Wang Qiaoyun Wu Fengjie Yang Jing |
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| Institution: | Wang Qiaoyun1,Wu Fengjie2,Yang Jing1 (1 Basic Medical Department,Binzhou Medical College,Yantai 264003,2 Clinical Medical Department,Binzhou Medical College2,Binzhou 256603) |
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| Abstract: | Objective: To study the effect of scorpion venom fibrinolytic active peptides(SVFAPs)on the oxygen-glucose deprivation /reoxygenation(OGD/R)injured neuron in rat.Methods: Primary cultured rat's cortex neurons were made into OGD/R model cells.The cell viability was detected by MTT assay and lactate dehydrogenase releasing methods;the activity of superoxide dismutase(SOD),and contents of malondialdehyde(MDA) and nitride oxide(NO) in culture supernate were detected;the apoptosis rate of cortex neurons after OG... |
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| Keywords: | scorpion venom fibrinolytic active peptides(SVFAPs) cortex neurons oxygen-glucose deprivation /reoxygenation |
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