| 芳香开窍药对脑缺血再灌注损伤大鼠保护作用机制的研究 |
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| 引用本文： | 倪彩霞,曾南,苟玲,刘金伟,何力,黄西芳,王建,夏厚林. 芳香开窍药对脑缺血再灌注损伤大鼠保护作用机制的研究[J]. 中药药理与临床, 2011, 0(5): 65-68 |
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| 作者姓名： | 倪彩霞 曾南 苟玲 刘金伟 何力 黄西芳 王建 夏厚林 |
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| 作者单位： | 成都中医药大学药学院教育部重点实验室中药标准化实验室; |
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| 基金项目： | 国家重点基础研究发展计划项目(No.2007CB512606) |
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| 摘 要： | 目的:研究麝香、冰片、安息香和苏合香四味芳香开窍药对脑缺血再灌注损伤大鼠的保护作用机制。方法:线栓法制备局灶性脑缺血再灌注损伤大鼠模型,观察药物对大鼠术前、缺血2h再灌注4h、10h、22h时体温的影响;比色法测定大鼠缺血2h再灌注22h后血清NO含量与NOS活性;ELISA法测定缺血侧脑组织中IL-1β和IL-6含量。结果:冰片(0.2g/kg)能明显抑制大鼠缺血2h再灌注4h、10h、22h时体温的异常升高,麝香(66.6mg/kg)和苏合香(1.332g/kg)仅在再灌注后4h时明显抑制体温升高,安息香(1.0g/kg)作用不明显。麝香、冰片和苏合香能显著降低模型动物血清NO含量,麝香、冰片还能显著抑制血清NOS活性。冰片、麝香能显著降低缺血侧脑组织IL-1β、IL-6含量,苏合香和安息香的作用不明显。结论:芳香开窍药对脑缺血再灌注损伤大鼠有一定的保护作用,其中麝香、冰片作用为优,其次苏合香,安息香作用较弱,作用机制可能与抑制炎症介质释放减轻炎症损伤及抗NO神经毒性有关。冰片对模型动物体温升高的良好抑制作用,从一个侧面反映了该药区别于其他三味药物的"寒凉"之性。
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| 关 键 词： | 芳香开窍药 脑缺血再灌注损伤 白细胞介素-1β 白细胞介素-6 一氧化氮 一氧化氮合酶 |
Protective mechanism of aromatic resuscitation drugs in cerebral ischemia-reperfusion injury model rats |
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| Ni Caixia,Zeng Nan,Gou Ling,Liu Jinwei,He Li,Huang Xifang,Wang Jian,Xia Houlin. Protective mechanism of aromatic resuscitation drugs in cerebral ischemia-reperfusion injury model rats[J]. Pharmacology and Clinics of Chinese Materia Medica, 2011, 0(5): 65-68 |
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| Authors: | Ni Caixia Zeng Nan Gou Ling Liu Jinwei He Li Huang Xifang Wang Jian Xia Houlin |
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| Affiliation: | Ni Caixia,Zeng Nan,Gou Ling,Liu Jinwei,He Li,Huang Xifang,Wang Jian,Xia Houlin (Pharmacy College,Chengdu University of Traditional Chinese Medicine,Chengdu 611137) |
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| Abstract: | Objective: To study the protective mechanism of four aromatic resuscitation drugs(moschus,borneolum,benzoinum,styrax) on cerebral ischemia-reperfusion injury in rats.Methods: Focal middle cerebral artery occlusion(MCAO) model was made by suture-occluded method.The rectal temperature of rats was observed on preoperative and reperfusion 4h,10h,22h after ischemia 2h.After 22h reperfusion,all animals were decapitated.The content of NO and NOS activity in serum were measured by chromatometry,and the content of I... |
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