| 秃疮花提取物对CCl4诱导的小鼠急性肝细胞微粒体损伤的影响 |
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| 引用本文： | 毛爱红,王勤,王廷璞,夏晓慧,魏娟,王明辉. 秃疮花提取物对CCl4诱导的小鼠急性肝细胞微粒体损伤的影响[J]. 中药药理与临床, 2011, 0(1): 73-75 |
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| 作者姓名： | 毛爱红 王勤 王廷璞 夏晓慧 魏娟 王明辉 |
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| 作者单位： | 甘肃省医学科学研究院;兰州大学生命科学学院; |
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| 基金项目： | 甘肃省兰州科技局科研基金资助课题 |
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| 摘 要： | 目的:探讨研究秃疮花提取物(DLE)对CCl4诱导的小鼠急性损伤肝细胞微粒体的影响。方法:小鼠灌胃给予不同剂量的DLE,连续2周。最后一次给药24h后CCl4造成急性肝损伤。制备肝细胞微粒体悬液,检测抗氧化酶SOD、CAT、GSH-Px的活性;测量肝细胞微粒体悬液中的MDA水平;观察NADPH-细胞色素C(P450)还原酶活性及ATPase活性的变化。结果:与模型组相比,不同剂量的DLE给药组肝细胞微粒体悬液中MDA含量显著减少;抗氧化酶SOD、GSH-Px活性显著增加;肝细胞微粒体NAD-PH-细胞色素C(P450)还原酶活性降低,Na+K+-ATPase和Ca2+-ATPase活性显著上升。结论:DLE对CCl4诱导的小鼠肝微粒体损伤具有很好的保护作用。其作用机理与DLE具抗氧化和自由基活性清除有关。
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| 关 键 词： | 秃疮花 急性肝损伤 肝细胞微粒体 |
Protection of Picranostigma leptopodum extraction against acute hepatic microsome injuries induced by carbon tetrachloride |
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| Mao Aihong ,,Wang Qin ,Wang Tingpu ,Wei Juan ,Xia Xiaohui ,Wang Minghui. Protection of Picranostigma leptopodum extraction against acute hepatic microsome injuries induced by carbon tetrachloride[J]. Pharmacology and Clinics of Chinese Materia Medica, 2011, 0(1): 73-75 |
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| Authors: | Mao Aihong Wang Qin Wang Tingpu Wei Juan Xia Xiaohui Wang Minghui |
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| Affiliation: | Mao Aihong 1,2,Wang Qin 1*,Wang Tingpu 1,Wei Juan 1,Xia Xiaohui 1,Wang Minghui 1 (1 School of Life Sciences,Lanzhou University,Lanzhou 730000,2Institute of Medical Sciencesof Gansu Province,Lanzhou 730050) |
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| Abstract: | Objective: This study examined the effect of DLE on hepatic microsome injury,from the aspect of lipid peroxidation after CCl 4 administration in mice.Methods: The activities of antioxidant enzymes of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px) in liver plasma and Na +,K +-ATPase,Ca 2+-ATPase in hepatic microsome suspension were detected respectively by its kits.Spectrophotography measured the activities of NADPH-cytochrome C(P450) reductase in microsome.The levels of malondialdehy... |
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