非霍奇金淋巴瘤中细胞凋亡与细胞增殖的研究

目的了解非霍奇金淋巴瘤(NHL)中细胞凋亡与细胞增殖间的关系,探讨两者在NHL发生与发展中的作用.方法利用TdT介导的dUTP缺口末端标记(TUNEL)法和PCNA免疫组化技术原位检测60例NHL中的细胞凋亡和增殖水平,计算凋亡指数(AI)和增殖指数(PI).结果B细胞性NHL中,随着恶性度增高,AI和PI均增高(P＜0.05),T细胞性NHL中高度恶性组PI明显高于低度恶性组(P＜0.05),而AI在两组之间无显著性差异(P＞0.05).AI和PI呈显著正相关(r=0.704,P＜0.01).结论在NHL中细胞凋亡与增殖之间可能存在密切的联系,共同参与了NHL的发生和恶性进展.对细胞凋亡和增殖水平的检测可能有助于NHL恶性度及预后的判断.

Study on cell apoptosis and proliferation of non-Hodgkin's lymphoma

Objective To investigate the relationship between cell apoptosis and proliferation and their roles in oncogenesis and progression of non Hodgkin's lymphoma (NHL).Methods TdT mediated dUTP nick end labeling (TUNEL) and immunohistochemistry of PCNA were used to detect in situ the apoptotic cells and the proliferative cells in 60 cases of NHL,then apoptotic index (AI) and proliferative index (PI) were calculated.Results AI of B cell type NHLs and PI of both type NHLs increased with of malignancy grade ( P <0.05),whereas there was no significant difference of AI between low grade and high grade of Tcell type NHLs ( P >0.05).A significant correlation was found between AI and PI (r=0.704, P <0.01).Conclusion Cell apoptosis may have a close correlation with cell proliferation in NHL and they may participate in oncogenesis and progression of NHL.Detection of cell apoptosis and proliferation may be helpful in judging malignancy grade and prognosis of NHL.;