反义hTR治疗联合Tamoxifen增强对人胶质瘤细胞生长的抑制作用

目的探讨Tamoxifen单独或联合端粒酶反义RNA（hTR）治疗对胶质瘤细胞生长的抑制作用；方法Tamoxifen单独或联合hTR反义cDNA作用于人胶质瘤细胞系TJ905细胞．用MTT法、TRAP法、TUNEL法、免疫组化法等检测效果。结果Tamoxifen、反义hTR治疗都能抑制TJ905细胞生长，7d后细胞生存率分别为38．6％（TAM浓度为15mM）、52．8％，以二者联合应用抑制作用最强，细胞生存率为20．6％；凋亡指数明显增加，胶质瘤细胞内端粒酶活性、PKC和IGF-1的表达被抑制。结论hTR反义治疗联合Tamoxifen能通过抑制PKC和IGF-1表达增强对胶质瘤细胞生长的抑制作用。

Antisense hTR combined with Tamoxifen increase the inhibitory effect on growth of glioma cells

Objective To study the inhibitory effect of Tamoxifen and/or antisense hTR cDNA on glioma cells. Method The cultured human glioblastoma TJ905 cells were treated with Tamoxifen and/or antisense hTR cDNA,their inhibitory effect was evaluated by MTT assay, TRAP method, TUNEL method, immunohistochemistry. Results Tamoxifen or antisense hTR cDNA alone can inhibit the growth of glioma cells significantly,after 7 days, rate of survival of TJ905 cell was 38.6% (concentration of TAM was 15mM) , 52.8%,respectively. But the inhibitory effect was strongest during combined treatment with Tamoxifen and antisense hTR cDNA, The rate of survival of cell was 20.6%. Apoptotic index increased significant. Expressions of PKC and IGF-1 and telomerase activity were inhibited. Conclusions Antisense hTR combined with Tamoxifen can increase inhibitory effect on growth of glioma cells through inhibiting expression of PKC and IGF-1.