血管性痴呆小鼠海马神经元ERK1 mRNA表达特征的研究

目的：探讨血管性痴呆（vascular dementia,VD）小鼠海马CA1区神经元ERK1 mRNA的表达及意义。方法：双侧颈总动脉线结反复缺血-再灌注法制备VD小鼠模型,设正常组、假手术组及血管性痴呆模型组,利用跳台试验和水迷宫试验观测其行为学改变,应用原位杂交技术观测其海马CA1区神经元ERK1 mRNA的表达变化。结果：VD模型组小鼠学习、记忆成绩低于正常组和假手术组（P〈0.05）。与正常组（34.79±21.10）和假手术组（34.43±18.65）比较,VD模型组小鼠海马CA1区的ERK1 mRNA阳性锥体细胞面密度值（14.57±3.67）明显减少（P〈0.01）。结论：VD小鼠学习、记忆成绩下降可能与其海马ERK1 mRNA表达水平增加有关。ERK1 mRNA的表达增加是血管性痴呆小鼠学习、记忆成绩下降的分子学机制之一。

Study of Expression of ERK1 mRNA in Neuron of Hippocampus in Mice with Vascular Dementia

Objective：To study the significance of expression of ERK1 mRNA in neuron of hippocampus CA1 in VD mice.Methods：The mice were subjected for ischemia and reperfusion repeatedly by the ligation of bilateral common carotid arteries to establish the VD models.The behavioral changes were investigated by step down test and water maze test.The Change of expression of ERK1 mRNA was observed with in situ hybridization.Results：The ability of learning and memory in VD mice had a significance lower than that in normal group and sham group（P〈0.05）,Compared with the normal group（34.79±21.10） and sham group（34.43±18.65）,the expression of ERK1 mRNA in neuron of hippocampus CA1 in VD mice（14.57±3.67） was also decreacer than them （P〈0.01）.Conclusion：The decreace of expression of ERK1 mRNA in neuron of hippocampus CA1 in VD mice is one of the molecule mechanism of degressive of learning and memory in VD mice.