蜂胶体外诱导人喉癌细胞Hep-2凋亡的实验研究

目的:探讨蜂胶对喉癌细胞珠Hep-2凋亡的影响及作用机制。方法:应用MTT、透射电镜、原位末端标记法观察药物对细胞的抑制率及凋亡形态、凋亡率变化。结果:MTT测得细胞抑制率随时间、剂量而增大,透射电镜下见到凋亡细胞及凋亡小体形成,原位末端标记检测出细胞凋亡率亦有时间、剂量依赖性。结论:蜂胶能够抑制喉癌细胞生长,其抑制作用是通过诱导喉癌细胞凋亡而起作用的。

Experimental study:Human laryngeal carcinoma cells apoptosis induced by propolis

Objective:To investigate the induction of apoptosis and its possible mechanisms in propolis-treated human laryngeal squamous cell carcinoma strain Hep-2.Methods:MTT,electronmicroscope,terminal deoxynudeotidyl transferrased UTP nick and labeling(TUNEL)were conducted to observe apoptosis index and apoptosis morphous.Results:Inhibition rate increased with time and dose,which were measured by MTT.There were dose-effect relationship among all samples.Apoptosis cells and apoptosis bodies were found under electronmicroscopy.Apoptosis index increased with time and dose dependence by TUNEL.Conclusion:Propolis can inhibit human laryngeal squamous cell arcinoma strain Hep-2.It`s inhibitive action may be through inducing human laryngeal carcinoma cells apoptosis.