成都地区151例健康成人血清HDL亚类组成的研究

目的：研究健康成人HDL亚类组成的相对含量及年龄、性别对HDL亚类组成的影响。方法：采用双相电泳－免疫印迹法分析了151例健康成人血清preβ－HDL及α－HDL各亚类含量。结果：健康成人血清preβ1-HDL,preβ2-HDL,HDL3c,HDL3b,HDL3a,HDL2a与HDL2b含量分别为6．11％、4．74％、5．97％、11．70％、20．27％、21．85％和29．19％。随年龄增长，健康成人血清中preβ1-HDL含量逐渐升高，HDL2b含量逐渐降低，其中男性50－59岁和≥60岁组，女性≥60岁组preβ1-HDL含量显著高于＜40岁组（P＜0．05）；男性及女性50－59岁和≥60岁组HDL2b含量显著低于＜40岁组（P＜0．01）。女性血清中preβ1-HDL含量较男性显著降低，而HDL2b含量显著正相关（P＜0．01），HDL2b含量与年龄（P＜0．01）、TG水平（P＜0．05）、apeB100水平（P＜0．05）呈显著负相关，与HDL－C水平呈显著正相关（P＜0．01）。结论：随着年龄的增长，健康成人血清HDL亚类颗粒呈逐渐变小的趋势，且男性HDL颗粒较女性小，胆固醇逆向转运过程有减弱的倾向。

Subclasses of Serum HDL in 151 Healthy adults in Chengdu

OBJECTIVE: To investigate the components of subclasses of serum HDL in healthy adults and the impact of age and gender on these components. METHODS: The components of subclasses of serum HDL in 151 healthy adults were determined by two dimensional gel electrophoresis associated with immunodetection method. RESULTS: The average concentration of prebeta1-HDL, prebeta2-HDL, HDL(3c), HDL(3b), HDL(3a), HDL2a, and HDL(2b) were 6.11%, 4.74%, 5.97%, 11.70%, 20.27%, 21. 85%, and 29.19%, respectively. The prebeta1-HDL increased with age, whereas the HDL(2b) decreased. The components of prebeta1-HDL in men over 50 and women over 60 were significantly greater than those of the same gender who were younger than 40, while the components of HDL2b in both men and women over 50 decreased compared to those of the same gender who were younger than 40. The component of prebeta1-HDL was significantly greater in men than in women, but it was reversed for HDL(2b). The prebeta1-HDL increased with the body mass index, age, and the concentration of TG. The HDL2b decreased with age and the concentrations of TG and apoB100. CONCLUSION: With the increase of age, there is a general shift toward smaller sized HDL, which indicates that the reverse cholesterol transport might be weakened. Men has smaller particle size than women.