逍遥丸对慢性束缚应激小鼠行为学和神经递质含量的影响

目的:考察逍遥丸对慢性束缚应激小鼠行为学和脑内神经递质含量的影响。方法:采用每天束缚小鼠8 h,连续10 d,制备慢性束缚应激小鼠模型,正常组和模型组ig给予等体积蒸馏水,药物组给予逍遥丸按人临床用量换算成小鼠等效剂量1.365 g.kg-1的混悬液,均在束缚前1 h ig给药,连续12 d,于第11 d采用旷场实验测定小鼠的自发活动,第12 d采用酶联免疫法测定小鼠不同脑区神经递质含量。结果:小鼠每天束缚8 h连续10 d后,穿格次数和直立次数显著减少(P<0.05),同时,海马5-羟色胺(5-HT)、前脑去甲肾上腺素(NE)、加压素(AVP)含量和下丘脑组胺含量非常显著增加(P<0.01)。连续ig给予逍遥丸,可显著增加模型小鼠的穿格次数(P<0.05),显著减少模型小鼠脑内的5-HT,NE,AVP含量(P<0.01)及组胺含量(P<0.05)。结论:逍遥丸明显改善慢性束缚引起的小鼠行为学异常,下调脑内单胺类神经递质和AVP含量变化。

Effect of Xiaoyao Wan on Behavior and Neurotransmitters in Mice with Chronic Restraint Stress

Objective: To investigate the effect of Xiaoyao Wan (XYW) on the behavior and the content of brain neurotransmitters in mice with chronic restraint stress (CRS), so as to provide some evidence for its clinical application. Method: Mice were restraint for 8 h daily over a period of 10 days to prepare the CRS model. Mice of control and model group were given orally with equal volume of distilled water, and XYW group was given suspension of XYW at the dose of 1.365 g·kg^-1 according to its clinical dose 1 h before restraint for 12 days. The locomotor activity of mice was observed in open field test (OFT) on the 11^th day and the content of neurotransmitters in various brain regions was examined by enzyme linked immunosorbent assay (ELISA) on the 12^th day. Result: Compared with the control group, both ambulation movements and rearing times in OFT were significantly decreased and the content of 5-hydroxytryptamine (5-HT) in hippocampus, norepinephrine (NE) and arginine vasopressin hormone (AVP) in forebrain and histamine in hypothalamus in CRS mice were significantly increased in the model group. XYW could significantly increase ambulation movements and rearing times in OFT and decrease the content of 5-HT, NE, AVP and histamine in brains of CRS mice. Conclusion: XYW could improve not only abnormal behavior but also alteration in content of monoamine neurotransmitters and AVP in brain induced by CRS.