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| 高效液相色谱法测定他米巴罗汀胶囊的含量 | |
| 引用本文： | 石涛,石滨,陈一辰.高效液相色谱法测定他米巴罗汀胶囊的含量[J].中国生化药物杂志,2011,32(4). |
| 作者姓名： | 石涛 石滨 陈一辰 |
| 作者单位： | 1. 东南大学附属中大医院 2. 南京医科大学附属妇幼保健院 3. 中国药科大学,江苏南京,210009 |
| 摘 要： | 目的建立他米巴罗汀胶囊含量测定方法。方法采用高效液相色谱法(HPLC)进行测定,色谱柱为CEC-C8柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸-甲醇(1∶1∶1),检测波长为235 nm。结果他米巴罗汀在2.51~40.12μg/mL浓度范围内,峰面积与浓度呈良好的线性关系,r=0.999 9;重复性试验的RSD为0.12%(n=6),最低检测限为0.2 ng,平均回收率为99.60%,他米巴罗汀主峰与辅料有良好分离。结论此法简单、灵敏、专属性强、重现性好,结果准确可靠,适用于他米巴罗汀胶囊的质量控制。 |
| 关 键 词： | 他米巴罗汀 高效液相色谱 含量测定 |
Assay of tamibarotene capsule by HPLC |
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| SHI Tao,SHI Bin,CHEN Yi-chen.Assay of tamibarotene capsule by HPLC[J].Chinese Journal of Biochemical Pharmaceutics,2011,32(4). | |
| Authors: | SHI Tao SHI Bin CHEN Yi-chen |
| Abstract: | Purpose To establish HPLC method for assay of tamibarotene capsule.Methods The sample of tamibarotene was determined by HPLC on the CEC-Cs column(150 mm×4.6 mm,5 μm).Results The standard curve of tamibarotene was linear in the range of 2.51-40.12 μg/mL,r=0.999 9,the RSD of repeatability test was 0.12%(n=6),the LOD was 0.2 ng,the average recovery was 99.60% and its RSD was 0.57%.The resolution between tamibarotene and excipient was good.Conclusion The method is simple,sensitive,accurate and reliable,which is an effective method for the determination of tamibarotene capsules. |
| Keywords: | tamibarotene HPLC assay |
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