| 不同主产地黑顺片的指纹图谱研究 |
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| 引用本文： | 黄慧莲,刘科兰,邵峰,任刚,段锋,刘荣华.不同主产地黑顺片的指纹图谱研究[J].中国实验方剂学杂志,2011,17(16):44-49. |
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| 作者姓名： | 黄慧莲 刘科兰 邵峰 任刚 段锋 刘荣华 |
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| 作者单位： | 江西中医学院现代中药制剂教育部重点实验室,南昌,330004 |
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| 基金项目： | 国家自然科学基金项目，国家中医药管理局中医药行业科研专项 |
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| 摘 要： | 目的:研究四川江油、陕西汉中及云南丽江等附子主产区黑顺片的高效液相色谱指纹图谱,为建立附子的行业质量标准提供依据。方法:利用HPLC方法,色谱条件Alltimate C18色谱柱(4.6 mm×250 mm,5μm),柱温35℃,流动相40mmol.L-1醋酸铵(氨水调pH 10.00)-乙腈梯度洗脱,流速1.0 mL.min-1,检测波长为240 nm。对不同主产地的22批黑顺片进行研究,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A和聚类分析对指纹图谱进行了分析。结果:22批黑顺片有11个共有峰,江油地区(道地产区)的黑顺片之间的相似度均大于0.900,非道地产区与道地产区的相似度小于0.900。结论:该方法精密度、重复性和稳定性良好,可结合黑顺片含量测定用于全面控制附子炮制品(黑顺片)的质量。
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| 关 键 词： | 黑顺片 高效液相 指纹图谱 |
| 收稿时间： | 2011/2/27 0:00:00 |
Studies on HPLC Fingerprint of Heishunpian from Different Origin |
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| HUANG Hui-lian,LIU Ke-lan,SHAO Feng,REN Gang,DUAN Feng and LIU Rong-hua.Studies on HPLC Fingerprint of Heishunpian from Different Origin[J].China Journal of Experimental Traditional Medical Formulae,2011,17(16):44-49. |
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| Authors: | HUANG Hui-lian LIU Ke-lan SHAO Feng REN Gang DUAN Feng and LIU Rong-hua |
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| Institution: | Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China;Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China;Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China;Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China;Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China;Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China |
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| Abstract: | |
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| Keywords: | Heisshunpian HPLC fingerprint |
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