毛蚶多肽提取物的体外免疫活性

目的考察毛蚶多肽提取物(PEAS)的体外免疫活性。方法 MTT法测定PEAS对小鼠脾淋巴细胞增殖的影响及对小鼠NK细胞杀伤活性的影响;中性红吞噬法测定PEAS对小鼠腹腔巨噬细胞吞噬功能的影响;流式细胞术测定PEAS所致小鼠脾淋巴细胞周期的变化;双抗体夹心ELISA法测定PEAS对主要细胞因子IFN-γ和IL-4分泌水平的影响。结果 PEAS能够显著促进小鼠脾淋巴细胞增殖(P<0.05或0.01),能够增强小鼠腹腔巨噬细胞吞噬中性红活性和小鼠NK细胞杀伤活性(P<0.05或0.01);PEAS能够促进脾淋巴细胞G0/G1期向DNA合成期(S期)转化;PEAS协同Con A作用,能够增加IFN-γ的分泌(P<0.05或0.01),并且能够抑制IL-4的分泌(P<0.05或0.01)。结论 PEAS体外可促进小鼠脾淋巴细胞、腹腔巨噬细与NK细胞的免疫功能,其机制可能与促进脾淋巴细胞DNA合成,增加IFN-γ的分泌量有关。

Immunomodulatory activity of polypeptides extract from Arca subcrenata Lischke(PEAS) in vitro

Purpose To study the immunomodulatory activity of polypeptides extracts from Arca subcrenata Lischke(PEAS) in normal mice in vitro.Methods The splenic lymphocytes proliferation and the NK killing activity were assessed by MTT method;the phagocytic ability of peritoneal macrophage(PMΦ) was evaluated with neutral red phagocytosis assay;the changes in the cell cycle of lymphocytes were detected with flow cytometry(FCM) and the level of IFN-γ and IL-4 was measured by ELISA methods.Results The results showed that PEAS could significantly promote the proliferation of lymphocytes(P<0.05 or P<0.01),and PEAS could also enhance the phagocytic ability of PMΦ and the cytotoxicity of natural killer(NK) cells remarkably(P<0.05 or P<0.01).PEAS could facilitate the entry of lymphocytes from the G0/G1 phase to the S phase;PEAS combined with Con A,could increase the level of IFN-γ,while decrease the level of IL-4(P<0.05 or P<0.01).Conclusion The results indicated that PEAS could improve the activities of mouse spleen lymphocytes,PMΦ and NK cells.It showed enhancement effect on the immune function of mice in vitro,which may be related to the increase in S phase cells and level of IFN-γ.