蝇蛆壳聚糖降血脂作用机制探讨

目的研究蝇蛆壳聚糖降血脂作用机制。方法利用以高脂饲料喂养大鼠诱导建立的饮食性高脂血症模型,采用灌胃不同剂量蝇蛆壳聚糖给药,通过生化法检测各组大鼠载脂蛋白、相关酶及活性物质,初步探讨蝇蛆壳聚糖调节血脂机制。结果蝇蛆壳聚糖能升高载脂蛋白AI(ApoAI),升高ApoAI/ApoB比值,使卵磷脂胆固醇酰基转移酶(LCAT)活性显著升高,提高超氧化物歧化酶(SOD)活性,降低丙二醛(MDA)含量,提高NO水平(P<0.01)。结论蝇蛆壳聚糖调节血脂的作用机制是多方面的。蝇蛆壳聚糖通过升高高密度脂蛋白胆固醇,升高ApoAI,激活LCAT,促进胆固醇的逆向转运,抑制低密度脂蛋白胆固醇氧化修饰,调节血脂水平。通过提高SOD活性,增强机体抗脂质过氧化作用的能力,调节血脂水平。通过促进NO的生成和释放,改善血管内皮的功能,调节血脂水平。

Research on mechanism of housefly maggot chitosan's regulation activity on blood lipid

Purpose To study the mechanism of housefly maggot chitosan′s(HMC) regulation activity on blood lipid.Methods The diet-induced hyperlipidemia model was established by using high-cholesterol diets.Then the rats were respectively fed by gavage with HMC at different doses.Contents of apolipoproteins,correlated enzymes and active substances were tested by biochemical methods.Results HMC could increase apolipoprotein AI(ApoAI)(P<0.01)and ApoAI/ApoB(P<0.01).It could activate lecithin cholesterol acyl transferase(LCAT)(P<0.01).It could activate SOD and lower MDA content(P<0.01).It could advance the NO level.Conclusion The mechanism of blood-fat-lowering effects is miscellaneous.It may be as follows:increasing HDL-C and ApoAI,activating LCAT and SOD and promoting NO.Therefore HMC can advance antiporting of cholesterol,strengthen anti-lipid peroxidation and improve the function of blood vessel endothelium.