Neu-p12 Neu-p68 Neu-p150对胰岛素抵抗3T3-L1脂肪细胞的葡萄糖摄取的影响

目的:在细胞水平,从Neu-p12、Neu-p68、Neu-p150中筛选可以改善胰岛素抵抗的药物。方法:采用"鸡尾酒"法,诱导分化出成熟的3T3-L1脂肪细胞,300μM油酸(OA)作用6 h建立胰岛素抵抗的细胞模型。实验分为5组,对照组、10 nM褪黑素组、10 nM Neu-p12组、10 nM Neu-p68组和10 nM Neu-p150组,采用2-脱氧-[3H]-D-葡萄糖的摄取实验,测定进入细胞内的葡萄糖的差异。结果:加药组的葡萄糖摄取率分别增加了508%、740%、499%、316%。结论:10 nM Neu-p12、10 nM Neu-p68、10 nM Neu-p150都可以促进胰岛素抵抗的3T3-L1脂肪细胞对葡萄糖的吸收,并且10 nM Neu-p12的效果最好。

Effect of Neu - pl2, Neu - pi 50 and Neu - p68 on glucose intake of insulin resistance 3T3 - L1 adipocytes

Objective:We screen wich drugs can improve insulin resistance among Neu-p12、 Neu-p68、and Neu-p150 at a cellular level.Methods: Mature 3T3-L1 adipocytes were differentiated by "cocktail" method,and were treated with 300 μM oleic acid(OA) for 6 hours to establish insulin resistance cell model.Five groups of adipocytes were employed:the control group,the 10 nM melatonin treatment group,the 10 nM Neu-p12 treatment group,the 10 nM Neu p68 treatment group,and the 10 nM Neu-p150 treatment group.Then we determined the differences of glucose into adipocytes of all groups,using 2——deoxyd-glucose uptake experiment.Results: The treatment groups increased respectively 508%、740%、499%、316%,of the counts of per minute.Conclusion: 10 nM Neu-p12,10 nM Neu-p68,and 10 nM Neu-p150 all can promote 3T3-L1 adipocytes to uptake glucose,and 10 nM Neu-p12 has the best effect.