血水草血根碱对钉螺肝脏损伤的研究

目的分析血水草血根碱（SAN）对钉螺肝脏糖原、重要酶活性及脂质过氧化的影响,探讨SAN对钉螺肝脏损伤的机理。方法自血水草粉末中分离SAN,配制5 mg/L水溶液,每500 ml溶液投放50只钉螺,25℃浸泡36 h,解剖活螺,分离肝脏,另设清水对照组。测定钉螺肝脏糖原和丙二醛（MDA）含量以及丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、超氧化物岐化酶（SOD）、过氧化物酶（POD）的活性,采用独立样本t检验对统计结果进行分析。结果 SAN组和清水对照组钉螺的肝脏糖原含量分别为（12.151±0.204）mg/g和（18.113±0.163）mg/g,差异有统计学意义（P〈0.05）;MDA水平分别为（5.298±0.441）nmol/mgprot和（4.351±0.197）nmol/mgprot,差异无统计学意义（P〉0.05）;SAN组钉螺的肝脏ALT、AST、ACP、AKP、SOD分别为（2.760±0.076）U/mg-prot、（68.723±2.295）U/mgprot、（407.949±19.868）U/gprot、（191.287±0.771）U/gprot、（48.452±0.193）U/mgprot,清水对照组钉螺分别为（1.104±0.000）U/mgprot、（49.448±1.626）U/mgprot、（344.475±30.186）U/gprot、（121.905±3.127）U/gprot、（38.814±2.765）U/mgprot,两组差异均具有统计学意义（P均〈0.05）,SAN组和清水对照组钉螺肝脏POD活性分别为（22.170±0.018）U/mgprot、（21.747±0.264）U/mgprot,两组POD差异无统计学意义（P〉0.05）。结论 SAN可引起钉螺肝脏糖原含量及一些重要酶活性的改变而导致钉螺肝功能损伤。

Study on liver injury of Oncomelania hupensis caused by Eomecon chinanthe sanguinarine

Objective To analyze the effects of Eomecon chinanthe sanguinarine（SAN） on glucogen,enzyme activity and lipid peroxidation of Oncomelania hupensis liver so as to explore the mechanism of SAN against Oncomelania hupensis.Methods SAN was extracted and purified from the dry powder of Eomecon chionantha.Oncomelania hupensis were immersed in 5 mg/L sanguinarine（50 Oncomelania hupensis per 500 ml solution） or clean water at 25 ℃ for 36 h,the livers were isolated from live snails.Total glucogen content,malondialdehyde（MDA） level,activities of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,acid phosphatase（ACP）,alkaline phosphatase（AKP）,superoxide dismutase（SOD）,peroxidase（POD） were determined respectively and the data were analyzed by independent t test.Results The glucogen content of snail livers in the SAN group and the control group were（12.151±0.204） and（18.113±0.163） mg/g respectively,the difference between the two groups was significant（P〈0.05）;the MDA levels of the two groups were（5.298±0.441） and（4.351±0.197） nmol/mgprot respectively,and the difference was not significant（P〉0.05）;the activities of ALT,AST,ACP,AKP,SOD in the SAN group were（2.760±0.076） U/mgprot,（68.723±2.295） U/mgprot,（407.949±19.868） U/gprot,（191.287±0.771） U/gprot and（48.452±0.193） U/mgprot respectively,the activities of these enzymes in the control group were（1.104±0.000） U/mgprot,（49.448±1.626） U/mgprot,（344.475±30.186） U/gprot,（121.905±3.127） U/gprot and（38.814±2.765） U/mgprot respectively,the activities of ALT,AST,ACP,AKP and SOD were significantly increased after immersed in 5 mg/L SAN for 36 h,the differences were significant（All P values〈0.05）;yet the difference of POD between the SAN group （22.170±0.018） U/mgprot ]and the control group （21.747±0.264） U/mgprot] was not significant（P〉0.05）.Conclusion SAN can destroy liver functions of Oncomelania hupensis through decreasing glucogen content and changing activities of some important enzymes in snail liver.