| 人参炔醇对氧糖剥夺神经细胞损伤的保护作用 |
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| 引用本文： | 段贤春,夏伦祝,汪永忠,程卉,章俊如.人参炔醇对氧糖剥夺神经细胞损伤的保护作用[J].中国实验方剂学杂志,2011,17(16):180-183. |
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| 作者姓名： | 段贤春 夏伦祝 汪永忠 程卉 章俊如 |
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| 作者单位： | 1. 安徽中医学院第一附属医院,国家中医药管理局中药制剂三级实验室,合肥,230031
2. 安徽中医学院科研实验中心,合肥,230031 |
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| 基金项目： | 安徽省中医药管理局中医药科研课题，安徽中医学院临床科学研究基金项目，康缘中医药科技创新基金项目 |
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| 摘 要： | 目的:探讨人参炔醇对氧糖剥夺(OGD)致神经细胞损伤的保护作用。方法:体外培养PC12细胞,分为正常对照组、OGD组、人参炔醇组、尼莫地平组,加入含0.5 mmol.L-1 Na2SO4的无糖Earle’s液建立OGD损伤模型,以人参炔醇(10,20mg.L-1)、尼莫地平(100μmol.L-1)处理。采用荧光显微镜观察细胞形态,Hoechst和PI双染流式细胞分析仪检测凋亡率,测定培养上清液中乳酸脱氢酶(LDH)的活性与细胞Ca2+浓度,观察人参炔醇对OGD致PC12细胞损伤的保护作用。结果:人参炔醇能够减少OGD损伤PC12细胞LDH的释放(P<0.05,P<0.01),抑制Ca2+的过量产生(P<0.05,P<0.01)、显著减少细胞凋亡及坏死(P<0.05,P<0.01)。结论:人参炔醇对OGD致神经细胞损伤具有保护作用。
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| 关 键 词： | 脑缺血 氧糖剥夺 人参炔醇 神经保护 |
| 收稿时间： | 2011/3/25 0:00:00 |
Protective Effects of Panaxynol on Nerve Cells under Oxygen and Glucose Deprivation |
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| DUAN Xian-chun,XIA Lun-zhu,WANG Yong-zhong,CHEN Hui and ZHANG Jun-ru.Protective Effects of Panaxynol on Nerve Cells under Oxygen and Glucose Deprivation[J].China Journal of Experimental Traditional Medical Formulae,2011,17(16):180-183. |
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| Authors: | DUAN Xian-chun XIA Lun-zhu WANG Yong-zhong CHEN Hui and ZHANG Jun-ru |
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| Institution: | First Affiliated Hospital, Anhui College of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine, Hefei 230031, China;First Affiliated Hospital, Anhui College of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine, Hefei 230031, China;First Affiliated Hospital, Anhui College of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine, Hefei 230031, China;Experimental Center, Anhui College of Traditional Chinese Medicine, Hefei 230031, China;First Affiliated Hospital, Anhui College of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine, Hefei 230031, China |
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| Abstract: | |
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| Keywords: | cerebral ischemia oxygen glucose deprivation panaxynol neuroprotective effect |
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