两种佐剂对日本血吸虫重组抗原SjGST-Sj32保护效果的影响

目的 比较弗氏完全佐剂（ＦＣＡ）与单磷脂Ａ（ＭＰＬ）的免疫增强作用，为提高重组ＳｊＧＳＴ－Ｓｊ３２（ｒＳｊＧＳＴ－Ｓｊ３２）的疫苗效果提供依据。方法 ｒＳｊＧＳＴ－Ｓｊ３２加弗氏完全佐剂（ｒＳｊＧＳＴ－Ｓｊ３２＋ＦＣＡ）或单磷脂Ａ（ｒＳｊＧＳＴ－Ｓｊ３２＋ＭＰＬ）免疫小鼠３次，ＥＬＩＳＡ法检测抗体水平，用减虫率及减卵率表示保护性作用。结果 与ＰＢＳ对照组相比，ｒＳｊＧＳＴ－Ｓｊ３２加ＦＣＡ或ＭＰＬ

SIGNIFICANT PROTECTION AGAINST SCHISTOSOMA JAPONICUM INDUCED BY RECOMBINANT FUSION PROTEIN SJGST-SJ32 WITH FCA OR MPL

Aim To compare the immunoenhancing effects between two adjuvants,Freund's complete adjuvant (FCA)and monophosphoryl lipid A (MPL), in vaccmation of mice with recombinant fusion protein SjGST-SJ32 (rSjGST-Sj32)Methods Mice were immunized three times with rSjGST-Sj32 plus FCA or MPL.Levels of specific antibodies were detected by ELISA.After challeng, the mice were perfused,the worms and the eggs which collected from the livers of mice were counted. Resulte significant levels of specific antibody were induced in mice immunized wth rSjGST-Sj32 plus either FCA or MPL.As compared with the control group, the worm reduction rates were 49% and 44% respectively; the liver egg pergram (LEPG)were 59% and 46% respectively, and the liver egg perfemale (LEPF) reduction rates were 35% and 19% respectively. Conclusion The results show that FCA or MPL plus rSjGSTSj32 could significantly induce protective immunity, however,the former induced significantly anti-fecundity immunity, but the latter had not such an effect.Adjuvant MPL was not as good as FCA in enhancing humoral immune response induced by rSjGST-Sj32 in mice.