2003年上海地区疑似狂犬的病毒检测及其意义的探讨

目的]对上海地区2003年疑似狂犬进行病毒检测，并探讨其意义。方法]采集90份犬脑标本，用ELISA法检测病毒抗原、小鼠感染法(MIT)行病毒分离、免疫荧光法(IFA)鉴定型别，并对咬人犬进行地区与时间分布调查。结果]咬人犬的阳性率为21．2％(11／52)，经IF法鉴定为Ⅰ型狂犬病毒；3种方法结果一致；非咬人犬均阴性；6～9月检出阳性犬最多，占全年的90．9％。阳性犬集中在嘉定和宝山。结论]检测狂犬病毒3种方法各有作用，必不可少。有病毒阳性犬的地区，应及时采取防治措施。

Rabies Virus Detection and its Significance of Suspect Dog in Shanghai,2003

Objective To investigate and detect rabies virus for suspect dogs and explore its significance in Shanghai in 2003. Brain samples of 90 dogs were collected and detected. Rabies virus antigen was detected by ELISA,virus was isolated by MIT and virus type was identified by IF. And the distribution of area and time were surveyed for suspect dogs. The positive rate of biting dogs was 21.2%(11/52)and the virus antigen belong to Type-I by IFA McAb. The results by 3 methods were identical. And non-biting dogs showed negative. The positive dogs accounted for 90.9% (10/11) from June to September and the dogs with rabies virus were mostly distributed in Jiading and Baosan districts. Conclusion ] The 3 methods for the virus isolation and identification are all needed. Rabies control and prevention should be carried out in the areas with positive dogs.