问接酶联免疫吸附法定量检测大鼠尿液水通道蛋白-2浓度

目的通过间接酶联免疫吸附测定（ELISA）法，检测正常大鼠不同水代谢状态下尿液水通道蛋白-2（AQP2）。方法收集大鼠正常，禁水24h以及水负荷状态（80ml／kg）下的尿液，用间接ELISA法检测尿液中AQP2。结果本方法批内差异系数6．5％，批间差异系数7％，灵敏度为112．5pmol／L；禁水24h后大鼠尿液AQP2／肌酐显著增加（P〈0．01），给予水负荷后尿液AQP2／肌酐显著降低（P〈0．01）；且尿渗量与尿液AQP2／肌酐呈正相关（r=0．952，P〈0．001）。结论间接ELISA法可以较好地检测大鼠尿液中AQP2。

Quantitative measurement of urinary excretion of aquaporin-2 water channel protein in rat by indirect ELISA

Objective To develop an efficient and stable enzyme-linked immunosorbent assay(ELISA) for measuring urinary aquaporin-2(AQP2) water channel protein.Methods Twenty-four hour urine of Sprague-Dawley rats was collected in various conditions of water metabolism and measured by indirect ELISA.Results The ELISA method could detect as low as 112.5 pmol/L AQP2 with intra-and inter-assay coefficients of variation(CVs) being 6.5% and 7% respectively.After dehydration for 24 hours,urinary AQP2/creatinine(Cr) increased(P<0.01).Subsequently,the rats were given oral water loading(80 ml/kg) and urinary AQP2/Cr significantly decreased(P<0.01).Also,urinary AQP2/Cr was positively correlated with urine osmolarity.Conclusion Indirect ELISA suitable for urinary AQP2 detection was established.