In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions |
| |
Authors: | Deniz Ortaç Turan Karaca Mehmet E Büyükokuro?lu Zafer Ö Özdemir Ay?e Tuba Kocaman |
| |
Institution: | 1. Department of Bioengineering, Biochemistry Division, Faculty of Chem. and Met. Eng., Y?ld?z Technical University, Istanbul, Turkey;2. Department of Histology and Embryology, Faculty of Medicine, University of Trakya, Edirne, Turkey;3. Department of Pharmacology, Faculty of Medicine, Sakarya University, Sakarya, Turkey;4. Technology Faculty, K?rklareli University, K?rklareli, Turkey |
| |
Abstract: | Context: Okra, Abelmoschus esculentus (L.) (Malvaceae), is a medicinal plant widely used in Turkish traditional medicine for the treatment of various diseases such as ulcers and gastritis.Objective: In the present study, we evaluated the gastroprotective effect of okra against ethanol-induced acute gastric mucosal injury in animal models.Materials and methods: Wistar rats were treated with 500, 250 or 100?mg/kg okra; 20?mg/kg famotidine (Fam); and 75?mg/kg quercetin (Que). Following a 60?min period, all the rats were given 1?mL of ethanol (80%). One hour after the administration of ethanol, all groups were sacrificed.Results: At 5000?mg/kg, the extract produced (okra) no signs of toxicity in animals. Okra 500, 250, 100, Fam 20 and Que 75 inhibited ulcer formation by 81.0, 67.5, 67.0, 76.3 and 72.4%, respectively. Okra 500 significantly decreased edema, hemorrhage and inflammation scores compared with the ethanol group (p?0.05). The oxidant levels decreased significantly in the all study groups compared within ethanol group (p?0.001). Serum β-carotene and retinol levels significantly increased 40.2 and 45.4% in the okra 500 group. In okra 500, 250 and Fam 20 groups, apoptosis significantly decreased (p?0.001), while okra 500, 250 and Fam 20 groups showed a higher percentage of cell proliferation compared with the ethanol group (p?0.001).Discussion and conclusions: Our in vivo data indicate that okra has a gastroprotective effect against ethanol and could reduce the gastric ulcer as seen from biochemical and histopathological results. We suggest that okra could be a possible therapeutic antiulcer agent. |
| |
Keywords: | Gastroprotection apoptosis gastric ulcer immunohistochemistry ulcer index ulcer inhibition |
|
|