| 多聚赖氨酸对胎鼠颌下腺细胞培养的影响 |
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| 引用本文： | 黄绍辉,张恩礁,周青,王玉新,徐颖. 多聚赖氨酸对胎鼠颌下腺细胞培养的影响[J]. 中国医科大学学报, 2006, 35(5): 510-511 |
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| 作者姓名： | 黄绍辉 张恩礁 周青 王玉新 徐颖 |
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| 作者单位： | 1. 中国医科大学附属口腔医院口腔颌面外科,辽宁,沈阳,110002
2. 鞍山市中心医院口腔科 |
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| 摘 要： | 目的：研究以多聚赖氨酸为生长底物对体外培养胎鼠颌下腺上皮细胞生长的影响。方法：以多聚赖氨酸包被培养板，对胎鼠颌下腺上皮细胞进行分离培养，应用相差显微镜及电镜观察细胞生长特性，免疫组织化学方法对细胞来源进行鉴定。结果：颌下腺细胞在包被有多聚赖氨酸的培养皿表面生长分化良好，呈单层生长；免疫组织化学检测单克隆角蛋白、上皮膜抗体呈阳性表达。结论：多聚赖氨酸在体外培养的过程中，可以促进细胞的黏附与生长。
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| 关 键 词： | 多聚赖氨酸 颌下腺细胞 细胞培养 |
| 文章编号： | 0258-4646(2006)05-0510-02 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2005-12-21 |
Effect of polylysine on embryonic rat submandibular gland cells cultured in vitro |
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| HUANG Shao-hui,ZHANG En-jiao,ZHOU Qing,WANG Yu-xin,XU Ying. Effect of polylysine on embryonic rat submandibular gland cells cultured in vitro[J]. Journal of China Medical University, 2006, 35(5): 510-511 |
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| Authors: | HUANG Shao-hui ZHANG En-jiao ZHOU Qing WANG Yu-xin XU Ying |
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| Affiliation: | 1. Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital, China Medical University, Shenyang 110002, China; 2. Department of Slomatology, Anshan Central Hospital |
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| Abstract: | Objective: To study the effect of polylysine(PL)on the survival and outgrowth of embryonic rat submandibular gland cells cultured in vitro.Methods: The submandibular gland cells were isolated from embryonic rats and digested into dissociated cell suspension for culture with PL-coated substrate.The submandibular gland cells were observed under phase contrast microscope and electron microscope and identified by immunohistochemical staining.Results: The cultured cells were polygonal epithelial cells with high survival rate,and they were positively stained with Anti-Pan cytokeratin and Anti-EMA,which were epithelium-specific antibodies.Conclusion: PL could promote the attachment and growth of cells cultured in vitro. |
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| Keywords: | polylysine submandibular gland cell cell culture |
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