| HPLC法测定四季感冒片中连翘苷的含量 |
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| 引用本文： | 蔡俊安,郭鑫慧. HPLC法测定四季感冒片中连翘苷的含量[J]. 中国中医药信息杂志, 2009, 16(10): 51-52 |
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| 作者姓名： | 蔡俊安 郭鑫慧 |
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| 作者单位： | 河南百年康鑫药业有限公司,河南,郸城,477150 |
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| 摘 要： | 目的采用HPLC法测定四季感冒片中连翘苷的含量。方法采用外标一点法,Diamonsil ODS1 C18色谱柱,乙腈-水（25∶75）为流动相,流速为1.0 mL/min,检测波长为277 nm。结果连翘苷在0.112 8～0.902 4μg范围内呈良好的线性关系,回归方程为Y=5.795 68X＋1.843 19,r=0.999 3,平均加样回收率为98.6%,RSD=1.82%（n=6）。结论本法简便、准确,专属性强,测定结果重复性好,为四季感冒片中连翘苷定量分析提供了有效的方法。
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| 关 键 词： | 四季感冒片 连翘苷 高效液相色谱法 含量测定 |
Determination of Phillyin in Siji Ganmao Plan by HPLC |
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| CAI Jun-an,GUO Xin-hui. Determination of Phillyin in Siji Ganmao Plan by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2009, 16(10): 51-52 |
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| Authors: | CAI Jun-an GUO Xin-hui |
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| Affiliation: | (Henan Bainian Kangxin Pharmaceutical CO.,Ltd, Dancheng 477150, China) |
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| Abstract: | Objective To establish the method for detemining the content of Phillyin in Siji Ganmao Pian by HPLC.Methods Diamonsil ODS1 C18 column was used with acetonitrile-water（25∶75） as the mobile phase,detection wavelength as 277 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.1128～0.9024 μg for Phillyin and linear equation was Y =5.795 68X ＋1.843 19,r =0.999 3.The average recovery was 98.6% and RSD was 1.82%（n =5）.Conclusion This method was simple,accurate and proper, the reduplication of the result was good. The method can be provide for quantitative analysis of Phillyin in Siji Ganmao Pian. |
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| Keywords: | Siji Ganmao Pian Phillyin HPLC content determination |
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