mRNA差异显示法比较独角莲作用肝癌细胞SMMC-7721前后的基因表达

目的 :研究独角莲根茎水提取物 (AEoTGE)作用肝癌细胞SMMC-7721后引起的细胞基因表达变化。方法 :利用mRNA差异显示技术 ,以人肝癌细胞系SMMC-7721细胞为研究对象 ,筛选AEoTGE作用后表达改变的基因片段。结果 :经测序和鉴定 ,发现 1个基因在加入AEoTGE后表达上升 ,1个基因表达下降。结论 :mRNA差异显示可以用于中草药对细胞的作用机制研究。

Detection of differentially expressed genes in hepatocellularcarcinoma cells SMMC-7721 treated with Typhonium giganteum extract by mRNA differential display

Objective: To screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC 7721 responsing to the aqueous extract from dried powdered rhizomes of Typhonium giganteum (AEoTGE) Method: The response of hepatocellular carcinoma cells SMMC 7721 to AEoTGE was explored with the technique of mRNA differential display Result: After hepatocarcinoma cells SMMC 7721 were treated by AEoTGE for 36 hours,1 gene expression was upgrade and 1 gene expression was downgrade induced by AEoTGE Conclusion: The research has provided important clues for the molecular mechanism of how hepatocarcinoma cells responseing to T giganteum.