Imaging of infection in rabbits with radioiodinated interleukin-1 (α and β), its receptor antagonist and a chemotactic peptide: a comparative study |
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Authors: | Conny J van der Laken Otto C Boerman Wim J G Oyen Marjo T P van de Ven Jos W M van der Meer Frans H M Corstens |
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Institution: | (1) Department of Nuclear Medicine, University Hospital Nijmegen, Nijmegen, The Netherlands, NL;(2) Internal Medicine, University Hospital Nijmegen, Nijmegen, The Netherlands, NL |
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Abstract: | Previous studies have reported the favourable characteristics of chemotactic peptides and interleukins for imaging of infection
and inflammation. In the present study, the potential of two species of interleukin 1 (IL-1), IL-1α and IL-1β, the IL-1 receptor
antagonist (IL-1ra) and the synthetic chemotactic peptide N-formyl-methionyl-leucyl-phenylalanyl-lysine (fMLFK) were directly compared in a rabbit model of infection. IL-1α, IL-1β,
IL-1ra and fMLFK were labelled with iodine-123 according to the Bolton-Hunter method. Twenty-four hours after induction of
Escherichia coli abscesses in the left thigh muscle, rabbits were injected intravenously with 0.5 mCi of 123I-labelled agent. Gamma camera images were obtained at 5 min and 1, 4, 8 and 20 h p.i. Biodistribution was determined at 20 h
p.i. Although all agents rapidly cleared from the blood, at 20 h p.i. blood levels and the levels in most organs of 123I-fMLFK were significantly lower than those of the other three agents (P<0.05). The abscesses were clearly visualized with all agents from 4 h p.i. onwards. After 1 h p.i., the abscess uptake of
123I-IL-1β was significantly higher than that of the other agents (P<0.05), with the highest uptake observed at 8 h p.i. (1.3%±0.3%). After 20 h p.i., the highest abscess-to-contralateral muscle
ratios were obtained with 123I-IL-1β, i.e. 39.0±11.5 vs 18.7±5.4, 18.1±2.3 and 29.9±7.0 for 123I-IL-1α, 123I-IL-1ra and 123I-fMLFK, respectively. In conclusion, all agents localized in the infectious focus. The potential of radiolabelled IL-1β for
imaging of infection was better than that of the other agents: higher absolute uptake in the infection and higher abscess-to-contralateral
muscle ratios were obtained. The observation of localization of radiolabelled IL-1ra in infection was important since this
protein can be administered to humans without any side-effects.
Received 11 October and in revised form 27 December 1997 |
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Keywords: | : Infection Chemotactic peptide Interleukin-1 f-Met-Leu-Phe Radioiodination |
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