低氧对大鼠睾丸部分生化指标影响

目的通过研究低氧对大鼠睾丸中部分生化指标的影响,探讨低氧对精子发生局部环境的影响。方法将成年雄性Wistar大鼠随机分为4组：常氧对照组、低氧5 d组、低氧15 d组和低氧30 d组。低氧各组置低氧舱内模拟5000 m高原减压。采用生化法测定各组大鼠睾丸组织中乳酸脱氢酶同工酶C4与乳酸脱氢酶的比值（LDH-C4/LDH）和γ-谷氨酰胺转移酶（GGT）活性,以及果糖、丙二醛含量。结果低氧30 d组大鼠睾丸组织LDH-C4/LDH显著低于常氧对照组（P〈0.05）;低氧30 d组GGT活性显著高于常氧对照组（P〈0.05）。低氧各组睾丸组织果糖含量与常氧对照组比较差异无统计学意义（P〉0.05）。低氧各组大鼠睾丸组织丙二醛含量均显著低于常氧对照组（P〈0.05）。结论低氧引起精子发生的环境改变,提示低氧影响精子的发生。

Changes of certain biochemical indexes of testis in rats exposed to hypoxia

Objective To explore the effects of hypoxia on the environment of spermatogenesis in rat.Methods Adult male Wistar rats were randomly divided into four groups:control group raised at sea level;5,15 and 30 d hypoxic group raised in a hypobaric chamber stimulating 5 000 m altitude for 5,15 and 30 days respectively.Lactate dehydrogenase C4/lactate dehydrogenase(LDH-C4/LDH),gamma-glutamyl transferase(GGT),fructose and malondialdehyde(MDA) were measured in testis.Results LDH-C4/LDH in hypoxic 30 d group was significantly lower than that in control group(P<0.05).Activity of GGT in hypoxic 30 d group was significantly higher than that in control group(P<0.05).Fructose level in testis was not significantly changed compared with control group.MDA decreased significantly when exposed to hypoxia(P<0.05).Conclusion Hypoxia changes the environment of spermatogenesis in rat.