葡萄籽原花青素预防大鼠动脉粥样硬化作用机制研究

目的在大鼠动脉粥样硬化（AS）模型发展过程中,应葡萄籽原花青素进行干预,探讨大鼠AS时葡萄籽原花青素对血总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）活性,及其抗动脉粥样硬化的作用机制。方法将64只健康Wistar雄性大鼠随机分为4组;A空白对照组（普通饮食）;B模型组（丙基硫氧嘧啶＋维生素D3＋高脂、高胆固醇饮食）;C：银杏黄酮组（90mg/kg,ig/d）;D：葡萄籽原花青素组（90mg/kg,ig/d）。10周后测定TC、TG、HDL、LDL活性。结果B组TC、TG、LDL,较A组明显升高（P〈0.01）,HDL降低（P〈0.01）;C组TC、TG、LDL、较B组显著降低（P〈0.01）,而HDL升高（P〈0.01）。D组的作用优于C组（P〈0.05）。结论葡萄籽原花青素具有降脂、阻止AS早期主动脉壁粥样硬化发展的作用。

Anti-atherosclerosis of procyanidin on experimental rat and its mechanisms

Objective To observe the effect of grape seed procyanidin （GSP） on the experimental early atherosclerosis in rats and its possible mechanisms.Methods 64 healthy male wistar rats were randomly divided into 4 groups：control group;model group;ginkgo flavone group（90 mg/kg,ig/d） and GSP（90 mg/kg,ig/d）.Rats in control group were fed with regular diet,and the model of atherosclerosis（AS） were fed with high lipid diet,propylthiouracil（PTU） and vitamin D3.After 10 weeks,serum was checked for the level of TG,TC,HDL-C,LDL-C.Results The levels of plasma TC,TG,LDL-C,were higher and that of HDL was lower in the model group compared with the control group（P〈0.01）.While compared with model group,The levels of plasma TC,TG,LDL,of each medication group was significantly lower （P〈0.01）,and that of HDL was higher compared with the model group（P〈0.01）,and GSP group was better than ginkgo flavone group（P〈0.05）.Conclusion GSP have potential effect to inhibit atherosclerosis formation,and its effect mechanism is related to its function of lipid-reduction.